Transcriptome of miRNA during inhibition of Bombyx mori nuclear polyhedrosis virus by geldanamycin in BmN cells

文献类型： 外文期刊

第一作者： Zhao, Zhi-meng

作者： Zhao, Zhi-meng;Yin, Hao-tong;Shen, Man-man;Zhang, Shao-lun;Chen, Zi-kang;Li, Tao;Zhang, Zhen-dong;Zhao, Wei-guo;Guo, Xi-jie;Wu, Ping;Shen, Man-man;Zhang, Zhen-dong;Zhao, Wei-guo;Guo, Xi-jie;Wu, Ping

作者机构：

关键词： BmNPV; HSP90 inhibitor; lncRNA-miRNA-mRNA network; microRNA; silkworm-pathogen interaction

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：2.454； 五年影响因子：2.11 ）

ISSN： 0739-4462

年卷期： 2022 年 110 卷 1 期

页码：

收录情况： SCI

摘要： Bombyx mori nuclear polyhedrosis virus (BmNPV) is one of several viruses that cause great harm to the sericulture industry, and its pathogenic mechanism is still being explored. Geldanamycin (GA), a kind of HSP90 inhibitor, has been verified to suppress BmNPV proliferation. However, the molecular mechanism by which GA inhibits BmNPV is unclear. MicroRNAs (miRNAs) have been shown to play a key role in regulating virus proliferation and host-pathogen interactions. In this study, BmN cells infected with BmNPV were treated by GA and DMSO for 72 h, respectively, then transcriptome analysis of miRNA was performed from the GA group and the control group. As a result, a total of 29 miRNAs were differentially expressed (DE), with 13 upregulated and 16 downregulated. Using bioinformatics analysis, it was found that the target genes of DEmiRNAs were involved in ubiquitin-mediated proteolysis, phagosome, proteasome, endocytosis pathways, and so on. Six DEmiRNAs were verified by quantitative reverse-transcription polymerase chain reaction. DElong noncoding RNA (DElncRNA)-DEmiRNA-messenger RNA (mRNA) regulatory networks involved in apoptosis and immune pathways were constructed in GA-treated BmN cells, which included 12 DEmiRNA, 132 DElncRNA, and 69 mRNAs. This regulatory network enriched the functional role of miRNA in the BmNPV-silkworm interactions and improved our understanding of the molecular mechanism of HSP90 inhibitors on BmNPV proliferation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序