Heterologous expression and characterization of a novel glycoside hydrolase family 55 beta-1,3-glucanase, AcGluA, from Archangium sp. strain AC19

文献类型： 外文期刊

第一作者： Wang, Yanxin

作者： Wang, Yanxin;Dong, Chaonan;Zhao, Yuqiang;Zhang, Lei;Yang, Fan;Ye, Xianfeng;Huang, Yan;Li, Zhoukun;Cui, Zhongli;Li, Ding;Zhao, Yuqiang;Cui, Zhongli

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关键词： beta-1,3-glucanase; Glycoside hydrolase family 55; Oligosaccharides; Immunostimulatory; Rice; Magnaporthe oryzae

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2021 年 105 卷 18 期

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收录情况： SCI

摘要： Some microbial-associated molecular patterns (MAMPs), like glucan oligosaccharides, can be recognized by pattern recognition receptors (PRRs) of plant to elicit further immunity response. In this study, a novel glycoside hydrolase family 55 beta-1,3-glucanase (AcGluA) from Archangium sp. strain AC19 was cloned and expressed in Escherichia coli. Among the reported beta-1, 3-glucanases from the glycoside hydrolase 55 family, the purified AcGluA exhibited the highest activity on laminarin at pH 6.0 and 60 degrees C with 112.3 U/mg. Activity of AcGluA was stable in the range of pH 4.0-9.0 and at temperatures below 60 degrees C. The K-m and V-max of AcGluA for laminarin were 3.5 mg/ml and 263.5 mu mol/(ml.min). AcGluA hydrolyzed laminarin into a series of oligosaccharides, suggesting it was an endo-beta-1,3-glucanase. The high dose of oligosaccharides (1600 mg/l) had conspicuous biocontrol efficacy on the defense of rice seedlings to Magnaporthe oryzae, which provided a new idea for the development of green biopesticide.

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