Purification, structural characteristics and anti-atherosclerosis activity of a novel green tea polysaccharide

文献类型： 外文期刊

第一作者： Zheng, Chao

作者： Zheng, Chao;Chen, Shun;Qian, Xin-Ping;Chen, Ying-Ying;Hong, Cheng-Zhi;Zeng, Ya-Fan;Li, Qiang-Ming;Pan, Li-Hua;Luo, Jian-Ping;Li, Xue-Ying;Zha, Xue-Qiang;Zheng, Chao;Chen, Shun;Qian, Xin-Ping;Chen, Ying-Ying;Hong, Cheng-Zhi;Zeng, Ya-Fan;Li, Qiang-Ming;Pan, Li-Hua;Luo, Jian-Ping;Li, Xue-Ying;Zha, Xue-Qiang;Deng, Yuan-Yuan

作者机构：

关键词： Green tea; Polysaccharide; Chemical structure; Anti-atherosclerosis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 254 卷

页码：

收录情况： SCI

摘要： A new homogeneous polysaccharide (TPS3A) was isolated and purified from Tianzhu Xianyue fried green tea by DEAE-52 cellulose and Sephacryl S-500 column chromatography. Structural characterization indicated that TPS3A mainly consisted of arabinose, galactose, galacturonic acid and rhamnose in a molar ratio of 5.84: 4.15: 2.06: 1, with an average molecular weight of 1.596 x 104 kDa. The structure of TPS3A was characterized as a repeating unit consisting of 1,3-Galp, 1,4-Galp, 1,3,6-Galp, 1,3-Araf, 1,5-Araf, 1,2,4-Rhap and 1-GalpA, with two branches on the C6 of 1,3,6-Galp and C2 of 1,2,4-Rhap, respectively. To investigate the preventive effects of TPS3A on atherosclerosis, TPS3A was administered orally to ApoE-deficient (ApoE-/-) mice. Results revealed that TPS3A intervention could effectively delay the atherosclerotic plaque progression, modulate dyslipidemia, and reduce the transformation of vascular smooth muscle cells (VSMCs) from contractile phenotype to synthetic phenotype by activating the expression of contractile marker alpha-smooth muscle actin (alpha-SMA) and inhibiting the expression of synthetic marker osteopontin (OPN) in high-fat diet-induced ApoE-/-mice. Our findings suggested that TPS3A markedly alleviated atherosclerosis by regulating dyslipidemia and phenotypic transition of VSMCs, and might be used as a novel functional ingredient to promote cardiovascular health.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序