A soybean EF-Tu family protein GmEF8, an interactor of GmCBL1 enhances drought and heat tolerance in transgenic Arabidopsis and soybean ,

文献类型： 外文期刊

第一作者： Zhang, Hui-Yuan

作者： Zhang, Hui-Yuan;Hou, Ze-Hao;Chen, Jun;Zhou, Yong-Bin;Chen, Ming;Fu, Jin-Dong;Ma, You-Zhi;Zhang, Hui;Xu, Zhao-Shi;Zhang, Yan;Li, Zhi-Yong

作者机构：

关键词： EF-Tu; Drought; Heat; Tolerant mechanism; Glycine max

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）

ISSN： 0141-8130

年卷期： 2022 年 205 卷

页码：

收录情况： SCI

摘要： A soybean elongation factor Tu family (EF-Tu) protein, GmEF8, was determined to interact with GmCBL1, and GmEF8 expression was found to be induced by various abiotic stresses such as drought and heat. An ortholog of GmEF8 was identified in Arabidopsis, a T-DNA knockout line for which exhibited hypersensitivity to drought and heat stresses. Complementation with GmEF8 rescued the sensitivity of the Arabidopsis mutant to drought and heat stresses, and GmEF8 overexpression conferred drought and heat tolerance to transgenic Arabidopsis plants. In soybean, plants with GmEF8-overexpressing hairy roots (OE-GmEF8) exhibited enhanced drought and heat tolerance and had higher proline levels compared to plants with RNAi GmEF8-knockdown hairy roots (MRGmEF8) and control hairy roots (EV). A number of drought-responsive genes, such as GmRD22 and GmP5CS, were induced in the OE-GmEF8 line compared to MR-GmEF8 and EV under normal growth conditions. These results suggest that GmEF8 has a positive role in regulating drought and heat stresses in Arabidopsis and soybean. This study reveals a potential role of the soybean GmEF8 gene in response to abiotic stresses, providing a foundation for further investigation into the complexities of stress signal transduction pathways.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序