Systematic analysis and functional verification of citrus glutathione S-transferases reveals that CsGSTF1 and CsGSTU18 contribute negatively to citrus bacterial canker

文献类型： 外文期刊

第一作者： Fu, Jia

作者： Fu, Jia;Fan, Jie;Yu, Qiyuan;Huang, Xin;Zhang, Chenxi;Xian, Baohang;Yang, Wen;Wang, Shuai;Chen, Shanchun;He, Yongrui;Li, Qiang;Su, Liyan;Chen, Shanchun;He, Yongrui;Li, Qiang;Chen, Shanchun;He, Yongrui;Li, Qiang

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关键词： Citrus; Glutathione S-transferases (GSTs); Citrus bacterial canker (CBC); Xanthomonas citri subsp. citri (Xcc); ROS homeostasis

期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：6.2； 五年影响因子：6.1 ）

ISSN： 2095-9885

年卷期： 2024 年 10 卷 6 期

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收录情况： SCI

摘要： Citrus bacterial canker (CBC) is resulted from Xanthomonas citri subsp. citri (Xcc) infection and poses a significant threat to citrus production. Glutathione S-transferases (GSTs) are critical in maintaining redox homeostasis in plants, especially in relation to abiotic and biotic stress responses. However, the function of GSTs in resisting CBC remains unclear. Here, citrus glutathione S-transferases were investigated applying a genome-wide approach. In total, 69 CsGSTs belonging to seven classes were identified, and the phylogeny, chromosomal distribution, gene structures and conserved motifs were analyzed. Several CsGSTs responded to Xcc infection, as observed in the upregulation of CsGSTF1 and CsGSTU18 in the CBC-sensitive 'Wanjincheng' variety but not in the resistant 'Kumquat' variety. CsGSTF1 and CsGSTU18 were localized at the cytoplasm. Transient overexpression of CsGSTF1 and CsGSTU18 mediated reactive oxygen species (ROS) scavenging, whereas the virus-induced gene silencing (VIGS) of CsGSTF1 and CsGSTU18 caused strong CBC resistance and ROS burst. The present study investigated the characterization of citrus GST gene family, and discovered that CsGSTF1 and CsGSTU18 negatively contributed to CBC through modulating ROS homeostasis. These findings emphasize the significance of GSTs in infection resistance in plants.

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