Genome-wide transcriptomic analysis of highly virulent African swine fever virus infection reveals complex and unique virus host interaction

文献类型： 外文期刊

第一作者： Ju, Xiaohui

作者： Ju, Xiaohui;Xiang, Guangtao;Zhao, Xiaomin;Ding, Qiang;Li, Fang;Zhao, Dongming;Li, Fang;Zhao, Dongming;Li, Jingrui;Wu, Chunyan;Nan, Yuchen;Ding, Qiang;Ding, Qiang

作者机构：

关键词： ASFV; PRRSV; Transcriptome analysis; Regulation network; miRNA; lncRNA; Innate immunity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2021 年 261 卷

页码：

收录情况： SCI

摘要： African swine fever virus (ASFV), one of the most devastating emerging swine pathogens in China, causes nearly 100 % mortality in naive herds. Here, whole-transcriptome RNA-seq analysis was conducted in porcine alveolar macrophages (PAMs) infected with Pig/Heilongjiang/2018 (Pig/HLJ/18) ASFV at different time points. Our data suggested that ASFV genes expression demonstrated a time-depended pattern and ASFV early genes were involved in antagonizing host innate immunity. Moreover, viral small RNA (vsRNA) was generated as well. Meanwhile, transcriptome analysis of host genes suggested a strong inhibition host immunity-related genes by ASFV infection in PAMs, while enhanced chemokine-mediated signaling pathways and neutrophil chemotaxis were observed in ASFV infected PAMs. Furthermore, ASFV infection also down-regulated host microRNAs (miRNAs) that putatively targeted viral genes, while also triggering dysregulation of host metabolism that promoted virus replication at transcription level. Most importantly, infection of PAMs with ASFV induced a different transcriptome pattern from that of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV), which is known to trigger a host cytokine storm. In conclusion, our transcriptome data implied that ASFV infection in PAMs appeared to be associated with strong inhibition of host immune responses, dysregulation of host chemokine axis and metabolic pathways.

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