Type-B response regulator RRB12 regulates nodule formation in Lotus japonicus

文献类型： 外文期刊

第一作者： Cao, Jingjing

作者： Cao, Jingjing;Zhou, Yu;Tian, Tao;Ji, Jie;Deng, Yan;Guan, Yuhao;Qi, Yongmei;Wang, Longxiang;Wang, Longlong;Huang, Yibo;Duanmu, Deqiang;Zhou, Yu;Wang, Longxiang;Wang, Longlong;Fan, Qiuling;Duanmu, Deqiang;Duanmu, Deqiang

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关键词： Cytokinin; Type-B response regulator; Nodule inception; Symbiotic nitrogen fixation; Infection thread; Nodule organogenesis

期刊名称：BMC BIOLOGY （ 影响因子：4.5； 五年影响因子：5.4 ）

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年卷期： 2024 年 22 卷 1 期

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收录情况： SCI

摘要： BackgroundThe mutualistic beneficial relationship between legume plants and rhizobia enables the growth of plants in nitrogen-limiting conditions. Rhizobia infect legumes through root hairs and trigger nodule organogenesis in the cortex. The plant hormone cytokinin plays a pivotal role in regulating both rhizobial infection and the initiation of nodule development. However, the mechanism used by the cytokinin output module to control symbiosis remains poorly documented.ResultsIn this study, we identified a cytokinin signaling output component encoded by the Type-B RESPONSE REGULATOR (RRB) gene, LjRRB12, which is expressed in Lotus japonicus nodule primordia and young nodules. Disruption of LjRRB12 leads to a reduction in nodulation and to an increase in the number of infection threads. Overexpression of LjRRB12D76E, an active form of the LjRRB12 protein, induces nodule-like structures in wild type and hit1 (hyperinfected 1/lotus histidine kinase 1) mutants but not in nin2 (nodule inception 2) mutants. Additionally, we utilized nCUT&Tag and EMSA to demonstrate that LjRRB12 can bind a CE (cytokinin response element) from the LjNIN promoter.ConclusionsOur results provide a deeper understanding of nodule organogenesis by establishing a link between the cytokinin signal and the transcriptional regulation of LjNIN.

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