Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin

文献类型： 外文期刊

第一作者： Liu, Y. -X.

作者： Liu, Y. -X.;Ma, C. -Y.;Ma, H. -Y.;Feng, C. -L.;Li, S. -J.;Ma, L. -B.;Liu, Y. -X.

作者机构：

关键词： Siganus oramin;Microsatellite marker;Polymorphism

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： Nine microsatellite DNA markers were developed and characterized for Siganus oramin by the 5' -anchored polymerase chain reaction technique. A total of 42 alleles were identified in 30 individuals, and the number of alleles per locus ranged from 3 to 7, with an average of 4.7. The observed and expected heterozygosity per locus ranged from 0.5333 to 1.0000 and from 0.5254 to 0.8474, respectively, with an average of 0.7422 and 0.6906, respectively. A significant deviation from the Hardy Weinberg equilibrium was detected at one microsatellite locus after a Bonferroni's correction (P < 0.0056). No significant linkage disequilibrium was found between any of the pairs of the nine loci. The microsatellite loci developed in this study will improve our understanding of the genetic background of S, oramin.

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