Identification of a novel immunological epitope on Hexon of fowl adenovirus serotype 4

文献类型： 外文期刊

第一作者： Liu, Jingyi

作者： Liu, Jingyi;Mei, Nan;Wang, Yaling;Shi, Xinjin;Chen, Hongjun

作者机构：

关键词： Fowl adenovirus; Hexon protein; Monoclonal antibody; Linear epitope

期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）

ISSN： 2191-0855

年卷期： 2021 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Fowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown. In this study, a monoclonal antibody (mAb) specific to Hexon protein of FAdV-4, designated as 3G8, was generated. Subsequently, the linear peptide recognized by 3G8 was mapped and identified as (213)AYGAYVK(219) using a series of overlapping peptides generated from Hexon protein. Amino acid sequence analysis revealed that the epitope recognized by 3G8 was highly conserved across all the FAdVs. The epitope was immunogenic and could be recognized by FAdV-4 positive chicken serum samples. These findings will enrich our knowledge regarding the epitope on Hexon and provide valuable information for further characterization of the antigenicity of Hexon protein.

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