Diagnosis and ORF Gene Sequencing Analysis of The Nervous Necrosis Virus (NNV) Isolated from Cultured Pearl Gentian Grouper, Epinephelus lanceolatus x Epinephelus fuscoguttatus, in China

文献类型： 外文期刊

第一作者： Ma, Rong-Rong

作者： Ma, Rong-Rong;Wang, Yin-Geng;Liao, Mei-Jie;Zhang, Zheng;Rong, Xiao-Jun;Li, Bin;Ma, Rong-Rong;Yang, Xian-Le

作者机构：

关键词： Grouper;NNV;RT-PCR;ORF sequencing;Phylogenetic analysis

期刊名称：2014 7TH INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING AND INFORMATICS (BMEI 2014)

ISSN：

年卷期： 2014 年

页码：

收录情况： SCI

摘要： Pearl gentian grouper, Epinephelus lanceolatus x Epinephelus fuscoguttatus, is a hybrid species with increasing market requirement in China. A disease outbreak occurred in the fish juvenile stages in Shandong Province, P.R. China. The gross signs were characterized by mass mortality, exhibiting dark skin coloration, reduce of feeding and having abnormal swimming behavior. Based on histopathological study, a large number of cellular vacuoles were observed in the diseased fish tissues of brain and retina. In addition, RT-PCR confirmed nervous necrosis virus (NNV) infection, and it was considered as the causative agent of the outbreak. This is, so far, the first report to detect NNV infection in Pearl gentian grouper. Furthermore, the open reading frames (ORF) of RNA dependent-RNA polymerase gene and coat protein gene of NNV also were sequenced after purification and cloning. Nucleotide sequence and the phylogenetic tree analysis of RNA1 and RNA2 showed extra high similarity (98.9%, 99.3% respectively) to red-spotted grouper nervous necrosis virus (RGNNV). These indicated that the NNV belong to RGNNV genotype, which is the only one genotype in P.R. China. Based on molecular study, the Cp genome included the ORF of 1017(6-1022) bases which encode protein of 338 amino-acids and the RdRp genome included the ORF of 2949 (49-2997) bases which encode protein of 982 amino-acids. These results would be helpful to understand NNV infection and provide information for fish health management.

分类号：

  • 相关文献

[1]The incidence and molecular characteristics of Apple stem grooving virus from pear in China. Hu, G. -J.,Wang, G. -P.,Wang, L. -P.,Hong, Ni.,Hu, G. -J..

[2]Detection and Sequence Analysis of Grapevine Leafroll-Associated Virus 2 Isolates from China. Fan, Xudong,Dong, Yafeng,Zhang, Zun Ping,Ren, Fang,Hu, Guojun,Zhou, Jun.

[3]Molecular characterization of the CP gene and 3 ' UTR of Chilli veinal mottle virus from South and Southeast Asia. Jan, F. -J.,Tsai, W. S.,Huang, Y. C.,Green, S. K.,Zhang, D. Y.,Reddy, K.,Hidayat, S. H.,Srithongchai, W.. 2008

[4]Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection. Tu, Jiagang,Chen, Wenjie,Zhao, Lijuan,Lan, Jiangfeng,Lin, Li,Fu, Xiaozhe,Lin, Qiang,Chang, Ouqin,Li, Ningqiu. 2016

[5]Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara. Liu, Xiao-Feng,Wu, Ya-Hong,Wang, Na,Li, Yang-Zhen,Zhang, Nian-Wei,Chen, Song-Lin,Liu, Xiao-Feng,Chen, Song-Lin,Liu, Xiao-Feng,Wei, Shi-Na,Li, Peng-Fei,Qin, Qi-Wei. 2018

[6]Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara. Liu, X. F.,Wu, Y. H.,Wang, N.,Li, Y. Z.,Zhang, N. W.,Chen, S. L.,Liu, X. F.,Chen, S. L.,Liu, X. F.,Wei, S. N.,Li, P. F.,Qin, Q. W.. 2018

[7]Molecular identification and functional analysis of Ctrp9 in Epinephelus coioides. Li, Wensheng,Qin, Chaobin,Wang, Bin. 2017

[8]Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China. Huang, X.,Huang, Y.,Wei, S.,Ouyang, Z.,Qin, Q.,Xu, L.,Feng, J..

[9]Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus x Epinephelus lanceolatus. Shen, G. M.,Shi, C. Y.,Fan, C.,Jia, D.,Wang, S. Q.,Xie, G. S.,Li, G. Y.,Mo, Z. L.,Huang, J.,Shen, G. M.,Shi, C. Y.,Fan, C.,Jia, D.,Wang, S. Q.,Xie, G. S.,Li, G. Y.,Mo, Z. L.,Huang, J.,Shen, G. M.,Fan, C.,Jia, D..

[10]Mass Mortality of Cage-cultured Orange-spotted Grouper Epinephelus coioides Associated with Renal Sphaerosporosis Caused by Sphaerospora epinepheli in South China Sea. Xu, Li-Wen,Feng, Juan,Zhang, Jing-Yong,Wang, Jian-Guo.

[11]Genetic variability in Grapevine virus A from Vitis vinifera L. x Vitis labrusca L. in Sichuan, China. Xi, Dehui,Yuan, Shu,Lin, Honghui,Wang, Jianhui,Chen, Keling,Li, Hongwen,Liu, Xiao,Wang, Jianhui,Liu, Jianjun,Chen, Keling,Li, Hongwen,Liu, Xiao,Liu, Jianjun,Ercisli, Sezai. 2012

[12]Rapid detection of porcine kobuvirus in feces by reverse transcription loop-mediated isothermal amplification. Li, Changlong,Chen, Jianfei,Shi, Hongyan,Zhang, Xin,Shi, Da,Han, Xiao,Feng, Li,Chi, Yanbin. 2014

[13]LMOf2365_0442 Encoding for a Fructose Specific PTS Permease IIA May Be Required for Virulence in L-monocytogenes Strain F2365. Liu, Yanhong,Yoo, Brian B.,Hwang, Cheng-An,Huang, Lihan,Suo, Yujuan,Sheen, Shiowshuh,Khosravi, Parvaneh. 2017

[14]Development of an RT-PCR detection method for mud crab reovirus. Guo, Zhi-Xun,Weng, Shao-Ping,Li, Guang,He, Jian-Guo,Guo, Zhi-Xun,Chan, Siu-Ming. 2008

[15]Protease-activated receptor-2 (PAR-2) regulates enterotoxigenic Escherichia coli-induced diarrhea during weaning in piglets. Pei, Xiao-ying,Guo, Ding-zong,Zhou, Dong-hai,Gao, Hong-Mei,Xia, Tian,Guo, Rui. 2010

[16]Rapid detection of newly isolated Tembusu-related Flavivirus by reverse-transcription loop-mediated isothermal amplification assay. Wang, Youling,Liao, Ming,Wang, Youling,Yuan, Xiaoyuan,Li, Yufeng,Yu, Kexiang,Yang, Jinxing,Xu, Huaiying,Zhang, Yuxia,Qin, Zhuoming,Yu, Kangzhen. 2011

[17]Estimating Plasmodiophora brassicae gene expression in lines of B. rapa by RT-PCR. Wu, Li-yan,Li, Shi-kai,Gong, Ya-ju,Zhong, Li,He, Jiang-Ming,Siemens, Johannes,Ludwig-Mueller, Jutta. 2012

[18]Cloning and expression analysis of a partial LEAFY homologue from pineapple (Ananas comosus (L.) Merr.). Lv Lingling,Sun Guangming,Zhang Jianxia,Zeng Songjun,Duan Jun,Lv Lingling,Xie Jianghui,Liu Shenghui,Liu Yuge,Wei Changbin,Lv Lingling,Sun Guangming. 2011

[19]Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum. Jian Gui-Liang,Zhang Ying-Tao,Ai Tie-Min.

[20]Identification and characterization of a novel adenine phosphoribosyltransferase gene (ZmAPT2) from maize (Zea mays L.). Wu, Suowei,Yu, Zhanwang,Li, Weihua,Yang, Qingkai,Ye, Chunjiang,Sun, Yan,Jin, Demin,Wang, Bin,Wu, Suowei,Li, Weihua,Yang, Qingkai,Wang, Fengge,Zhao, Jiuran.

作者其他论文 更多>>

微信小程序