Identification and functional study of detoxification-related genes in response to tolfenpyrad stress in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

文献类型： 外文期刊

第一作者： Pan, Xin

作者： Pan, Xin;Ding, Jian-Hao;Zhao, Shuai-Qi;Shi, Hui-Cong;Miao, Wang-Long;Wu, Fu-An;Sheng, Sheng;Zhou, Wei-Hong;Wu, Fu-An;Sheng, Sheng;Zhou, Wei-Hong

作者机构：

关键词： Glyphodes pyloalis; Tolfenpyrad; Transcriptome; Detoxification enzyme gene; RNA interference

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2023 年 194 卷

页码：

收录情况： SCI

摘要： Glyphodes pyloalis Walker (G. pyloalis) is a common destructive mulberry pest. Due to the long-term and frequent use of insecticides, it has developed tolerance to commonly used insecticides. Tolfenpyrad (TFP) is a novel pyrazole heterocyclic insecticide. In order to understand the TFP detoxification mechanism of G. pyloalis larvae, we first estimated the LC30 dose of TFP for 3rd instar G. pyloalis larvae. Next, we identified genes that were differentially expressed in 3rd instar G. pyloalis larvae treated with TFP compared to the control group by transcriptome sequencing. In total, 86,949,569 and 67,442,028 clean reads were obtained from TFP-treated and control G. pyloalis larvae, respectively. A total of 5588 differentially expressed genes (DEGs) were identified in TFP-treated and control G. pyloalis larvae, of which 3084 genes were upregulated and 2504 genes were down-regulated. We analyzed the expression of 43 candidate detoxification enzyme genes associated with insecticide tolerance using qPCR. According to the spatiotemporal expression pattern of DEGs, we found that CYP6ABE1, CYP333A36 and GST-epsilon8 were highly expressed in the midgut, while CarEs14 was strongly expressed in haemolymph. Furthermore, we successfully knocked down these genes by RNA interference. After silencing CYP6ABE1 and CYP333A36, bioassay showed that the mortality rate of TFP-treated G. pyloalis larvae was significantly higher compared to the control group. This study provides a theoretical foundation for under-standing the sensitivity of G. pyloalis to TFP and establish the basis for the effective and green management of this pest.

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