A high-throughput screening method for GM soybean events based on single universal primer multiplex PCR and capillary electrophoresis

文献类型： 外文期刊

第一作者： Dong, Liming

作者： Dong, Liming;Li, Congcong;Xing, Zhenjuan;He, Yuxuan;Xia, Wei;Long, Likun;Li, Feiwu;Liu, Yijun

作者机构：

关键词： Genetically modified soybean; High-throughput detection; Single universal primer multiplex PCR; Capillary electrophoresis

期刊名称：JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY （ 影响因子：1.4； 五年影响因子：1.7 ）

ISSN： 1661-5751

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Genetically modified (GM) crops and their products are regulated in many countries. To meet increasing regulatory requirements for a growing number of GM soybean events, high-throughput and cost-effective detection methods are indispensable. In this study, 14 widely commercialized GM soybean events, including GTS40-3-2, MON89788, CV127, A2704-12, A5547-127, 305423, 356043, MON87701, MON87705, MON87708, MON87769, SYHT0H2, FG72, and DAS-444 & Oslash;6-6 were utilized as targets for event-specific identification. A high-throughput detection method was established by integrating a single universal primer multiplex PCR (SUP-M-PCR) with capillary electrophoresis technique. This method enables the identification of 14 soybean events in a single PCR reaction, addressing the limitations of conventional multiplex PCR such as self-inhibition and amplification variations between different primers. Furthermore, it significantly enhances the sensitivity and accuracy of previous multiple detection methods, achieving a detection sensitivity of 0.05% (w/w). Overall, this approach greatly improves detection efficiency and holds promising potential for the identification and detection of 14 types of GM soybean events and their derivatives.

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