Overexpression of IbMPK3 increases low-temperature tolerance in transgenic sweetpotato

文献类型： 外文期刊

第一作者： Jin, Rong

作者： Jin, Rong;Liu, Ming;Zhao, Peng;Zhang, Aijun;Zhang, Qiangqiang;Liu, Yaju;Cao, Qinghe;Tang, Zhonghou;Kim, Ho Soo;Kwak, Sang-Soo;Yu, Tao;Yu, Wenhui;Yu, Wenhui

作者机构：

关键词： Low-temperature stress; Overexpression; IbMPK3; Sweetpotato

期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.496； 五年影响因子：2.406 ）

ISSN： 1863-5466

年卷期： 2022 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Sweetpotato is an important crop that is very sensitive to low temperatures. Mitogen-activated protein kinase (MAPK) is involved in plant growth and development and is responsive to many environmental stresses. IbMPK3 is strongly regulated by low-temperature stress. We studied the function of IbMPK3 and how it may enhance the adaptability of sweetpotato plants to low-temperature stress. Transgenic sweetpotato plants overexpressing IbMPK3 were generated, and three transgenic lines with the highest expression level of IbMPK3 were used for low-temperature tests. Transgenic plants exposed to low-temperature stress had less damage associated with higher photosynthesis efficiency and less cell membrane damage. IbMPK3-overexpressing transgenic plants could modulate reactive oxygen species (ROS) metabolism with lower levels of O-2(-) and H2O2 accumulation and higher enzymatic activities than WT plants. Transcript expression levels of some ROS-related genes (IbCAT, IbCu-ZnSOD, and IbCAT) and stress-responsive genes (IbP3B and IbCOR27) were significantly upregulated in transgenic plants compared to WT. These results indicate that IbMPK3 has an important role in sweetpotato response to low temperatures.

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