Stress-induced higher vein density in the C-3-C-4 intermediate Moricandia suffruticosa under drought and heat stress

文献类型： 外文期刊

第一作者： Zhu, Xiaoyi

作者： Zhu, Xiaoyi;Liu, Jun;Sun, Xingchao;Kuang, Chen;Liu, Hongfang;Zhang, Liang;Zheng, Qiwei;Liu, Jing;Li, Jun;Wang, Hanzhong;Hua, Wei;Liu, Jing;Hua, Wei

作者机构：

关键词： Abiotic stress; C-3-C-4 intermediate; C-4 anatomy; co-expression network; comparative transcriptome; genetic regulation; high vein density; Moricandia suffruticosa

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：7.298； 五年影响因子：8.291 ）

ISSN： 0022-0957

年卷期： 2022 年 73 卷 18 期

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收录情况： SCI

摘要： The C-3-C(4)intermediate Moricandia suffruticosahad higher leaf vein density than its C(3)relative and vein density was further increased by drought and heat, regulated by stress-inducible auxin signaling. The evolution of C-4 photosynthesis involved multiple anatomical and physiological modifications, yet our knowledge of the genetic regulation involved remains elusive. In this study, systematic analyses were conducted comparing the C-3-C-4 intermediate Moricandia suffruticosa and its C-3 relative Brassica napus (rapeseed). We found that the leaves of M. suffruticosa had significantly higher vein density than those of B. napus, and the vein density was further increased in M. suffruticosa under drought and heat stress. Moreover, the bundle sheath distance, as the mean distance from the outer wall of one bundle sheath to the outer wall of an adjacent one, decreased and the number of centripetal chloroplasts in bundle sheath cells was found to be altered in M. suffruticosa leaves under drought and heat treatments. These results suggest that abiotic stress can induce a change in an intermediate C-3-C-4 anatomy towards a C-4-like anatomy in land plants. By integrating drought and heat factors, co-expression network and comparative transcriptome analyses between M. suffruticosa and B. napus revealed that inducible auxin signaling regulated vascular development, and autophagy-related vesicle trafficking processes were associated with this stress-induced anatomical change. Overexpressing three candidate genes, MsERF02, MsSCL01, and MsDOF01, increased leaf vein density and/or enhanced photosynthetic assimilation and drought adaptability in the transgenic lines. The findings of this study may improve our understanding of the genetic regulation and evolution of C-4 anatomy.

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