Cyproheptadine hydrochloride inhibits African swine fever viral replication in vitro

文献类型： 外文期刊

第一作者： Cui, Huimei

作者： Cui, Huimei;Yang, Jinke;Yang, Bo;Hao, Yu;Shi, Xijuan;Zhang, Dajun;Zhang, Ting;Zhao, DengShuai;Yuan, Xingguo;Chen, Xuehui;Liu, Xiangtao;Zheng, Haixue;Zhang, Keshan

作者机构：

关键词： Periactin; African swine fever virus; Replication inhibition; Antiviral affect

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.8； 五年影响因子：4.0 ）

ISSN： 0882-4010

年卷期： 2023 年 175 卷

页码：

收录情况： SCI

摘要： African swine fever (ASF) is an infectious disease caused by the African swine fever virus (ASFV), and has a high mortality rate. It has caused serious socioeconomic consequences worldwide. Currently, there are no available commercial vaccines or antiviral drug interventions. D1133L is one of the key genes for ASFV replication and antiviral drug screening. In this study, a virtual screening software program, PyRx, was used to screen libraries of compounds against the potential drug target D1133L. Twelve compounds with a high affinity for ASFV D1133L were screened, and cyproheptadine hydrochloride (periactin) was identified as a candidate drug. The periactin has little cytotoxicity, and which dose-dependently inhibited ASFV replication in vitro. Further research indicated that periactin could significantly down-regulate D1133L at the transcriptional and protein levels with RT-qPCR and western blot methods. This study has provided important candidate drugs for the prevention and treatment of ASF, as well as biological materials and new fields of view for the research and development of vaccines and drugs for ASFV.

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