CUT&tag-seq analysis of heat stress response in broiler liver provides novel insights into the improved thermotolerance by dietary phloretin

文献类型： 外文期刊

第一作者： Hu, Hong

作者： Hu, Hong;Huang, Ying;Pan, Hongbin;Hu, Hong;Wang, Kunping;Bai, Xi;Chen, Liang

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关键词： Phloretin; Broilers; Liver; Heat stress; CUT&tag-seq; Nrf2-DNA sites interaction

期刊名称：ANNALS OF AGRICULTURAL SCIENCES （ 影响因子：5.9； ）

ISSN： 0570-1783

年卷期： 2023 年 68 卷 1 期

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收录情况： SCI

摘要： This work aimed to study the effect of phloretin on antioxidant profiles through nuclear factor erythroid 2- related 2 (Nrf2)-DNA interactions in the liver of heat-stressed broilers by Cleavage Under Targets and Tagmentation combined with high-throughput sequencing (CUT&Tag-seq). One hundred and sixty 22-day-old Arbor Acres broilers were divided into four groups: a normal temperature group (NT: 22.8-23.4 degrees C and 46-55.8 % humidity) was fed a basal diet and three high temperature groups (China Summer condition: 29.7-31.7 degrees C and 77.9-94.8 % humidity) were fed the basal diet supplemented with 0 (HT group), 100 mg/kg (LT group) and 200 mg/kg (PT group) phloretin. Liver samples were taken from 42-day-old broilers. High tem-perature condition increased (P <= 0.05) the malondialdehyde (MDA) level, but decreased (P <= 0.05) the activities and mRNA expression levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), and Nrf2. Dietary 200 mg/kg phloretin decreased (P <= 0.05) the MDA level, but increased (P <= 0.05) the activities and mRNA expression levels of GSH-Px, SOD, CAT and Nrf2 in the liver of heat-stressed broilers. CUT&Tag-seq showed that 4042 DEPs were up-regulated, while 2322 DEPs were down-regulated in the PT group compared with the HT group. The Nrf2-binding gene of DEPs contained SOD3, CAT, SOD2, and p38 mitogen-activated pro-tein kinase. Most TOP20 significant KEGG pathways were associated with "Environmental Information Process-ing". Summary, phloretin could improve oxidative damage by Nrf2 and its downstream DNA-sites including genes and Nrf2-bingding non-coding RNAs in the liver of heat-stressed broilers.(c) 2023 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).

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