Xyloglucan endotransglucosylase-hydrolase 22 positively regulates response to cold stress in upland cotton (Gossypium hirsutum L.)

文献类型： 外文期刊

第一作者： Wu, Hongmei

作者： Wu, Hongmei;Lian, Boying;Lv, Xiaoyan;Sun, Mengxi;Wei, Fei;An, Li;Fu, Xiaokang;Lu, Jianhua;Ma, Liang;Wang, Hantao;Wei, Hengling;Wu, Hongmei;Li, Yiran;Hao, Fushun

作者机构：

关键词： Upland cotton; GhXTH22; GhMYB44; Cold stress; ROS

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.6； 五年影响因子：5.7 ）

ISSN： 0926-6690

年卷期： 2024 年 220 卷

页码：

收录情况： SCI

摘要： Xyloglucan endotransglucosylase/hydrolases (XTHs) are cell wall modifying enzymes that function in response to multiple abiotic stresses by promoting cell wall reinforcement in plants. However, it is elusive whether GhXTH22 plays a role in cold stress tolerance in upland cotton (Gossypium hirsutum L.). In this study, we found that Arabidopsis (Arabidopsis thaliana (L.) Heynh) transgenic lines overexpressing GhXTH22 exhibit clearly increased cold tolerance compared with wild type (WT). Moreover, cold stress results in markable enhancements in the activities of catalase (CAT) and peroxidase (POD), cell wall rigidity, and the expression of cold responsive genes, as well as a notable reduction in malondialdehyde (MDA) levels in the overexpression (OE) lines compared with those in WT plants. Consistently, silencing GhXTH22 by virus-induced gene silencing (VIGS) method causes markedly decreases in cold stress tolerance in upland cotton. Additionally, by electrophoretic mobility shift assay (EMSA) and dual-luciferase assay, it was confirmed that GhMYB44 activate the expression of GhXTH22 by binding to AACNG motif on the promoter, and silencing the expression of GhMYB44 can decrease the cold resistance of cotton. In summary, this study systematically confirmed the cold resistance function of GhXTH22 and discovered the upstream regulating gene of GhXTH22, providing gene resources for cold resistance and enriching the knowledge on the mechanism of cold resistance.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序