R gene triplication confers European fodder turnip with improved clubroot resistance

文献类型： 外文期刊

第一作者： Yang, Zhiquan

作者： Yang, Zhiquan;Liu, Dongxu;Yin, Feifan;Wang, Jing;Yang, Qingyong;Jiang, Yingfen;Jiang, Yingfen;Gong, Jianfang;Li, Qian;Dun, Bicheng;Yuan, Lei;Zhou, Xueqing;Wang, Huiying;Shah, Nadil;Nwafor, Chinedu Charles;Chen, Peng;Wang, Bingrui;Zhou, Yongming;Li, Zaiyun;Zhang, Chunyu;Jiang, Yingfen;Gong, Jianfang;Li, Qian;Dun, Bicheng;Yuan, Lei;Zhou, Xueqing;Wang, Huiying;Shah, Nadil;Nwafor, Chinedu Charles;Chen, Peng;Wang, Bingrui;Zhou, Yongming;Li, Zaiyun;Zhang, Chunyu;Dun, Bicheng;Zhan, Zongxiang;Piao, Zhongyun;Zhou, Yuanwei;Zhu, Li;Li, Shisheng;Xiang, Jun;Zhu, Li;Li, Shisheng;Xiang, Jun

作者机构：

关键词： clubroot; turnip; CR gene; whole-genome triplication; functional analysis

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.263； 五年影响因子：11.619 ）

ISSN： 1467-7644

年卷期： 2022 年 20 卷 8 期

页码：

收录情况： SCI

摘要： Clubroot is one of the most important diseases for many important cruciferous vegetables and oilseed crops worldwide. Different clubroot resistance (CR) loci have been identified from only limited species in Brassica, making it difficult to compare and utilize these loci. European fodder turnip ECD04 is considered one of the most valuable resources for CR breeding. To explore the genetic and evolutionary basis of CR in ECD04, we sequenced the genome of ECD04 using de novo assembly and identified 978 candidate R genes. Subsequently, the 28 published CR loci were physically mapped to 15 loci in the ECD04 genome, including 62 candidate CR genes. Among them, two CR genes, CRA3.7.1 and CRA8.2.4, were functionally validated. Phylogenetic analysis revealed that CRA3.7.1 and CRA8.2.4 originated from a common ancestor before the whole-genome triplication (WGT) event. In clubroot susceptible Brassica species, CR-gene homologues were affected by transposable element (TE) insertion, resulting in the loss of CR function. It can be concluded that the current functional CR genes in Brassica rapa and non-functional CR genes in other Brassica species were derived from a common ancestral gene before WGT. Finally, a hypothesis for CR gene evolution is proposed for further discussion.

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