Integrated transcriptome and metabolome analysis of photoperiod effects on testosterone secretion in ChaHua chicken No.2 Roosters

文献类型： 外文期刊

第一作者： Liu, Mengqian

作者： Liu, Mengqian;Tan, Lei;Li, Xinlu;Li, Haojie;Zhang, Yong;Zi, Xiannian;Ge, Changrong;Wang, Kun;Liu, Mengqian;Liu, Mengqian

作者机构：

关键词： Transcriptome; Metabolome; light intensity; Chahua No.2 rooster; Testosterone

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 4 期

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收录情况： SCI

摘要： This study investigated the impact of light duration on Testosterone secretion in Chahua No.2 roosters, utilizing combined Transcriptome and Metabolome analyses to uncover critical genes, Metabolites, and signaling pathways. We randomly selected 240 Chahua No.2 roosters at 42 days old and divided them into four groups: simulated natural light (I), 12L:12D (II), 16L:8D (III), and 20L:4D (IV), each group has 6 replicates, with 10 chickens per replicate. Blood samples were collected at 91 and 140 days post-hatch to measure Testosterone levels. Results showed that at 91 days, group IV had significantly higher Testosterone levels than groups I, II, and III (P G 0.01), with group III also higher than groups I (P G 0.01) and II (P G 0.05). By 140 days, group IV maintained significantly higher Testosterone than groups I and II (P G 0.01) and higher than group III (P G 0.05), while group III was also elevated compared to groups I and II (P G 0.05). Testicles Transcriptomics analysis revealed 891 differentially expressed genes, including 479 down-regulated and 412 up-regulated genes. Key signaling pathways identified included Steroid Hormone Biosynthesis, Cytochrome P450, and retinol metabolism. Testicles Metabolomics analysis identified 174 differential Metabolites, with 91 up-regulated and 83 down-regulated, focusing on pathways like Amino Sugar Metabolism and Tryptophan Metabolism. Integrated analysis pinpointed 19 common signaling pathways, with the top ten including Cytochrome P450, tyrosine metabolism, and amino acid biosynthesis. Our findings indicate that extending light duration enhances Testosterone secretion in roosters. Through comprehensive transcriptomic and metabolomic analyses, we established that pathways associated with steroid hormone synthesis and Cytochrome P450 play a crucial role in light duration-regulated Testosterone secretion, highlighting key genes such as CYP11A1, CYP17A1, and HSD3B1, alongside Metabolites like ergosterol-5,7,22,24(28)-tetraene-3beta-alcohol.

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