The RNA-seq transcriptome analysis identified genes related to rice seed dormancy
文献类型: 外文期刊
第一作者: Xie, K.
作者: Xie, K.;Bai, J.;Yang, Y. Y.;Duan, N. B.;Ma, Y. M.;Guo, T.;Ding, H. F.;Yao, F. Y.;Ding, H. F.
作者机构:
关键词: differentially expressed genes; Oryza sativa; transcription factors
期刊名称:BIOLOGIA PLANTARUM ( 影响因子:1.747; 五年影响因子:2.146 )
ISSN: 0006-3134
年卷期: 2019 年 63 卷
页码:
收录情况: SCI
摘要: Plant hormones play important roles in seed dormancy and dormancy breaking. We measured the hormone content in rice (Oryza sativa L. cv. Nona Bokra) seeds at different stages and with or without imbibition treatment. We identified 1 265 differentially expressed genes (DEGs) between dormant and dormancy-broken seeds using RNA-seq analysis: 1 015 genes were significantly up-regulated, while 250 genes were significantly down-regulated. Sixteen DEGs were selected as related to seed dormancy, and their expressions were validated using quantitative PCR. Three DEGs were in the same position as two reported dormancy QTLs, suggesting that they may be candidate genes that control the dormancy of rice seeds. Our study provides an important basis for cloning genes in dormant rice seeds and provides theoretical support for the study of the dormancy mechanism.
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