Identification of candidate genes related to soluble sugar contents in soybean seeds using multiple genetic analyses

文献类型： 外文期刊

第一作者： Pan Wen-jing

作者： Pan Wen-jing;Huang Shi-yu;Yu Jing-yao;Zhao Ying;Qu Ke-xin;Zhang Ze-xin;Yin Zhen-gong;Qi Hui-dong;Yu Guo-long;Xin Da-wei;Zhu Rong-sheng;Liu Chun-yan;Wu Xiao-xia;Jiang Hong-wei;Hu Zhen-bang;Chen Qing-shan;Qi Zhao-ming;Han Xue;Zuo Yu-hu;Zhang Yong

作者机构：

关键词： soybean soluble sugar contents; consensus QTL; meta-overview-collinearity integrated analysis; population validation; RNA-seq and candidate gene mining

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.384； 五年影响因子：4.021 ）

ISSN： 2095-3119

年卷期： 2022 年 21 卷 7 期

页码：

收录情况： SCI

摘要： Soluble sugar content in seeds is an important quality trait of soybean. In this study, 57 quantitative trait loci (QTLs) related to soluble sugar contents in soybean seeds were collected from databases and published papers. After meta-overview-collinearity integrated analysis to refine QTL intervals, eight consensus QTLs were identified. To further verify the consensus QTLs, a population of chromosome segment substitution lines (CSSLs) was analyzed. Two lines containing fragments covering the regions of consensus QTLs and the recurrent parent were selected: one line showed high soluble sugar contents associated with a consensus QTL fragment, and the other line showed low soluble sugar contents. Transcriptome sequencing was conducted for these two lines at the early, middle, and late stages of seed development, which identified 158, 109 and 329 differentially expressed genes, respectively. Based on the analyses of re-sequencing data of the CSSLs and the consensus QTL region, three candidate genes (Glyma.19G146800, Glyma.19G122500, and Glyma.19G128500) were identified in the genetic fragments introduced from wild soybean. Sequence comparisons between the two CSSL parents SN14 and ZYD00006 revealed a single nucleotide polymorphism (SNP) mutation in the coding sequence of Glyma.19G122500, causing a non synonymous mutation in the amino acid sequence that affected the predicted protein structure. A Kompetitive allele-specific PCR (KASP) marker was developed based on this SNP and used to evaluate the CSSLs. These results lay the foundation for further research to identify genes related to soluble sugar contents in soybean seeds and for future soybean breeding.

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