Extraction, purification and anti-TMV effects of alpha (beta)-2,7,11-cembratriene-4,6-diol from tobacco leaves
文献类型： 外文期刊
第一作者： Yan, Bowen
作者： Yan, Bowen;Huang, Bin;Wang, Jing;Wang, Jie;Jia, Haijiang;Huang, Chongjun;Wei, Jinhong;Mao, Duobin;Huang, Shen
作者机构：
关键词： alpha-2,7,11-cembratriene-4,6-diol; beta-2,7,11-cembratriene-4,6-diol; TMV; Antiviral activity
期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.449； 五年影响因子：6.508 ）
ISSN： 0926-6690
年卷期： 2021 年 174 卷
页码：
收录情况： SCI
摘要： Acetone ethanol extracts from tobacco leaves have antiviral activity against tobacco mosaic virus (TMV), but the antiviral effects of their specialized metabolites have not been systematically studied yet, especially the underlying mechanism is still unexplored. The tobacco cembranoids alpha(beta)-2,7,11-cembratriene-4,6-diol (alpha(beta)-CBD) were extracted and purified with an effective protocol including innovatively added 5% phosphoric acid for elution, one time silica gel chromatographic column separation and impurity removal and further HPLC purification. The in vivo anti-TMV activities showed that alpha(beta)-CBD have higher protective effects of 73.2% and 71.6%, at 75.0 mu M, respectively, than control agent Ningnanmycin (53.1%). Notably, The results of ELISA and TMV-GFP fluorescent optical imaging assay indicated a obviously reduced viral protein and weaker GFP fluorescence signal and smaller infection area, which confirmed their antiviral effects at protein level. Furthermore, the enhanced production of SA and JA and the significantly increased transcription of JA (COI1 and PDF1.2) and SA (PR1, NPR1 and EDS1) signaling pathway genes in treated plants further conformed that exogenously applied alpha(beta)-CBD can effectively elicit the tobacco plant immunity against TMV. The alpha(beta)-CBD mainly stimulates disease resistance of tobacco plants to resist TMV and it can be used as bioagents to control TMV in the future.
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