Transepithelial Transport of Hemp Protein-Derived Bioactive Peptide Trp-Tyr-Thr (WYT) in Human Caco-2 Cell Monolayers

文献类型： 外文期刊

第一作者： Wei, Ping

作者： Wei, Ping;Ling, Dongning;Tang, Yayuan;Deng, Zhonglin;Wei, Zhen;Wei, Ping;Ling, Dongning;Tang, Yayuan;Liu, Guoming;Liu, Guoming

作者机构：

关键词： Hemp; Bioactive peptide; Caco-2 cell monolayers; Transepithelial transport; Permeability

期刊名称：INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS （ 影响因子：2.4； 五年影响因子：2.0 ）

ISSN： 1573-3149

年卷期： 2025 年 31 卷 4 期

页码：

收录情况： SCI

摘要： PurposeTo improve bioactive peptide absorption and bioavailability, in-depth understanding of their transport across the intestinal epithelium is crucial. In this study, we used Caco-2 cell monolayers to investigate the transepithelial transport of Trp-Tyr-Thr (WYT), a novel hemp protein-derived Angiotensin-I converting enzyme (ACE) peptide.MethodsFirstly, Caco-2 cell monolayer model was established and then various cell modulators (Gly-Pro, sodium azide, wortmannin and cytochalasin D) were used to study WYT transport.ResultsOur results demonstrated that WYT maintained its stability for more than 2 days and 2 h in Hank's balanced salt solution and on the apical surface of Caco-2 cells, respectively. WYT was transported intact across Caco-2 cell monolayers with an apparent permeability coefficient (Papp) of 1.03 x 10-7 cm/s. Our transport route evaluation revealed that Gly-Pro, a peptide transporter 1 (PepT1) substrate, significantly affected transepithelial permeability, suggesting that it mediated WYT transport across Caco-2 cell monolayers. Moreover, the ATPase inhibitor sodium azide significantly reduced the Papp of WYT, while the tight junction disruptor cytochalasin D and the transcytosis inhibitor wortmannin did not significantly affect WYT permeability.ConclusionIn summary, the results of our study indicate that WYT transport across Caco-2 cell monolayers occurs energy-dependently via the PepT1 pathway. It contributes to the study of hemp protein-derived bioactive peptide in lowering blood pressure and supports the development of bioactive peptide products.

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