Natural peptidoglycan nanoparticles enable rapid antigen purification and potent delivery of plant-derived vaccines

文献类型： 外文期刊

第一作者： Song, Shi-Jian

作者： Song, Shi-Jian;Diao, Hai-Ping;Meng, Hui-Xin;Guo, Yong-Feng;Kang, Sumin;Kim, Kwang Soon;Hwang, Inhwan;Zhang, Sen-Sen;Song, Shi-Jian;Liu, Chang;Kim, Seonghan;Yun, Jieun;Kim, Ki Hean;Moon, Hongju;Kim, Woe-Yeon

作者机构：

关键词： plant molecular pharming; Nicotiana benthamiana; bacterium-like particle; vaccine; adjuvant; pep; ti-doglycan; protein purification

期刊名称：PLANT COMMUNICATIONS （ 影响因子：11.6； 五年影响因子：11.8 ）

ISSN： 2590-3462

年卷期： 2025 年 6 卷 8 期

页码：

收录情况： SCI

摘要： Plant molecular farming is a promising platform for biopharmaceutical production, however, downstream processing remains a challenge due to cost and complexity. In this study, we present natural peptidoglycan nanoparticles (NPNs) derived from Gram-positive lactic acid bacteria as a novel tool for plant-based vaccine purification and delivery. Sequential treatment with trichloroacetic acid and trypsin effectively reduced NPN size, removing residual host subcellular constructs and proteins while preserving protein-binding capacity. Optimizing trimeric protein anchors and trimerization elements for plant-based expression enabled protein binding at low temperatures, minimizing proteolytic degradation. NPNs conjugated with plant-derived hemagglutinin elicited strong humoral immune responses in mice. Additionally, NPNs enhanced the retention of GFP at the injection site and supported efficient polyclonal antibody generation. These findings establish NPNs as a versatile platform for plant-based recombinant vaccine purification and delivery.

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