A sensitive bio-barcode immunoassay based on bimetallic Au@Pt nanozyme for detection of organophosphate pesticides in various agro-products

文献类型： 外文期刊

第一作者： Chen, Ge

作者： Chen, Ge;Cui, Xueyan;Wang, Yuanshang;Zheng, Weijia;She, Yongxin;Wang, Jing;Jin, Maojun;Chen, Ge;Liu, Guangyang;Xu, Donghui;Huang, Xiaodong;Jia, Huiyan;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Sun, Jianchun;Liu, Haijin;Zou, Yuting;Li, Dongyang;Jin, Maojun;Hammock, Bruce D.;Li, Dongyang;Jin, Maojun;Hammock, Bruce D.

作者机构：

关键词： Nanozyme; Bio-barcode; Organophosphate pesticides; Au@Pt; AuNPs

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2021 年 362 卷

页码：

收录情况： SCI

摘要： Organophosphate pesticides (OPs) are often used as insecticides and acaricides in agriculture, thus improving yields. OP residues may pose a serious threat, due to inhibition of the enzyme acetylcholinesterase (AChE). Therefore, a competitive bio-barcode immunoassay was designed for simultaneous quantification of organophosphate pesticide residues using AuNP signal amplification technology and Au@Pt catalysis. The AuNP probes were labelled with antibodies and corresponding bio-barcodes (ssDNAs), MNP probes coated with ovalbumin pesticide haptens and Au@Pt probes functionalized with the complementary ssDNAs were then prepared. Subsequently, pesticides competed with MNP probes to bind the AuNP probes. The recoveries of the developed assay were ranged from 71.26 to 117.47% with RSDs from 2.52 to 14.52%. The LODs were 9.88, 3.91, and 1.47 ng & sdot;kg- 1, for parathion, triazophos, and chlorpyrifos, respectively. The assay was closely correlated with the data obtained from LC-MS/MS. Therefore, the developed method has the potential to be used as an alternative approach for detection of multiple pesticides.

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