miRNA-125a regulates porcine oocyte maturation in vitro by targeting ADAR

文献类型： 外文期刊

第一作者： Han, Yue

作者： Han, Yue;Gu, Weiyu;Li, Chunyu;Lv, Yanqiu;Qu, Xinglin;Zhang, Yuyang;Xu, Qinglong;Yao, Shunfa;Chen, Xuan;Jin, Yi;Lu, Ping;Yu, Yongsheng

作者机构：

关键词： Extracellular vesicles; miR-125a; Oocyte quality; Adenosine deaminase RNA specific

期刊名称：THERIOGENOLOGY （ 影响因子：2.5； 五年影响因子：2.7 ）

ISSN： 0093-691X

年卷期： 2025 年 235 卷

页码：

收录情况： SCI

摘要： Follicular fluid extracellular vesicles are beneficial for in vitro oocyte maturation and development; however, their effect on the expression profiles of oocyte microRNAs (miRNAs) and the roles of related miRNAs are unknown. In this study, we aimed to investigate miRNA expression in mature oocytes cultured in follicular fluid extracellular vesicles and the effect of miRNA-125a (miR-125a) on oocyte maturation. The expression profiles of the miRNAs were determined by microRNA sequencing, followed by target gene prediction analysis. We transfected miR-125a mimics and an miR-125a inhibitor to evaluate the effect of modulated miRNA-125a on cumulus expansion, oocyte maturation rate, changes in cytoplasmic maturation-related indicators, and changes in the expression of oocyte maturation-related, cumulus expansion-related, and predicted target genes. We found that miR-125a overexpression decreased the levels of cumulus expansion-related, oocyte maturation-related, and predicted target genes, adenosine deaminase RNA specific (ADAR), and lipid droplet number, and it increased the percentage of oocytes with abnormal cortical granule distribution. Inhibiting miR-125a increased the expression levels of oocyte maturation-related and target genes, number of lipid droplets, and endoplasmic reticulum function, and it decreased lipid droplet size. Mitochondrial membrane potential and reactive oxygen species levels were not significantly different between groups. In conclusion, our results suggest that extracellular vesicles may improve oocyte quality by modulating ADAR through regulating miR-125a.

分类号：

  • 相关文献
作者其他论文 更多>>
  • The genetic basis and improvement of photosynthesis in tomato

    作者：Dong, Haiqiang;Li, Fangman;Xuan, Xiaoxiao;Ahiakpa, John Kojo;Tao, Jinbao;Zhang, Xingyu;Ge, Pingfei;Wang, Yaru;Gai, Wenxian;Zhang, Yuyang;Zhang, Yuyang;Zhang, Yuyang;Zhang, Yuyang

    关键词：Photosynthesis; Tomato; Genetics; Improvement; Leaf; Fruit

  • The wheat NLR pair RXL/Pm5e confers resistance to powdery mildew

    作者：Guo, Guanghao;Hou, Yikun;Zhang, Huaizhi;Lu, Ping;Li, Miaomiao;Dong, Lingli;Xie, Jingzhong;Zhu, Keyu;Li, Beibei;Dong, Lei;Yang, Yijun;Qiu, Dan;Wang, Gaojie;Zhao, Yusheng;Chen, Yuhang;Liu, Zhiyong;Guo, Guanghao;Ahn, Hee-Kyung;Zhao, He;Jones, Jonathan D. G.;Bai, Kaihong;Hou, Yikun;Li, Beibei;Dong, Lei;Liu, Zhiyong;Gong, Zhen;Han, Guan-Zhu;Wu, Qiuhong;Chen, Yongxing;Li, Wenling;Li, Hongjie;Zhang, Panpan;Yuan, Chengguo;Shi, Wenqi;Xue, Minfeng;Yang, Lijun;Yu, Dazao;Hu, Tiezhu;Liu, Zhiyong;Ahn, Hee-Kyung

    关键词：wheat; powdery mildew; NLR pair; hetero-complexes; resistance mechanism

  • Relationship between the CUBN and the MIA3 gene copy number variation and growth traits in different cattle breeds

    作者：Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian;Han, Yue;Liu, Jiwei;Zhang, Congcong;Sun, Ming;Li, Xuanyu;Liu, Hongliang;Li, Shengnan;Zhu, Yongchao;Li, Ruidong;Luo, Xiaotong;Zhao, Yumin;Wu, Jian

    关键词：Grassland red cattle; yanbian cattle; yan Yellow cattle; intrinsic factor-cobalamin receptor; MIA SH3 domain ER-derived factor 3; copy number variations

  • Insight into the enhancement and mechanism of saltiness perception by salty peptides from bovine bone

    作者：Wang, Haiyan;Lu, Wenjing;Chen, Di;Chen, Xuan;Zhang, Cen;Xiao, Chaogeng;Wang, Haiyan;Xiao, Chaogeng;Dang, Yali;Chen, Xuan;Gou, Zhongjun;Wang, Yongjun

    关键词：Salty peptides; TMC4; Sodium reduction; Stevens' law; Molecular dynamics simulation

  • IPM-AgriGPT: A Large Language Model for Pest and Disease Management with a G-EA Framework and Agricultural Contextual Reasoning

    作者：Zhang, Yuqin;Fan, Qijie;Chen, Xuan;Li, Fuzhong;Guo, Leifeng;Zhang, Yuqin;Fan, Qijie;Chen, Xuan;Li, Min;Guo, Leifeng;Zhao, Zeying

    关键词：large language models; pest and disease; chain-of-thought distillation; agriculture

  • Multiresidue analysis of pesticides and dietary risk assessment of Coix seed by UPLC-MS/MS

    作者：Chen, Yangxin;Huang, Min;Lu, Ping;Song, Bangyan;Chen, Jing

    关键词：UPLC-MS/MS; QuEChERS; Pesticide residues; Fungicide; Coix seed; Risk assessment

  • The differential partition of copper in cell wall and symplastic space contributes to the natural variation of copper toxicity tolerance in rice

    作者：Zhang, Jin;Qin, Yuan;Chen, Xuan;Xiao, Nayun;Jiang, Wei;Tang, Haiyang;Zhou, Hui;Qiu, Xianjin;Zeng, Fanrong;Deng, Fenglin;Xu, Jianlong;Chen, Zhong-Hua;Chen, Zhong-Hua;Chen, Guang;Deng, Fenglin

    关键词：Copper; Rice (Oryza Sativa); Root elongation; Cell wall; Transporter

微信小程序