MicroRNA-34-5p regulates the expression of ecdysteroid receptor (ECR) in the process of salivary gland degeneration of ticks

文献类型： 外文期刊

第一作者： Hu, Shanming

作者： Hu, Shanming;Wang, Yanan;Zhou, Yongzhi;Cao, Jie;Zhang, Houshuang;Zhou, Jinlin

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关键词： Salivary gland degeneration; Apoptosis; miR-34-5p; RhECR

期刊名称：PARASITES & VECTORS （ 影响因子：3.5； 五年影响因子：3.5 ）

ISSN： 1756-3305

年卷期： 2025 年 18 卷 1 期

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收录情况： SCI

摘要： BackgroundThe salivary glands of female ticks rapidly degenerate after feeding via programmed cell death mediated by an ecdysteroid receptor (ECR). The degeneration includes both apoptosis and autophagy. The process of degeneration can also be regulated by microRNAs (miRNAs), but the underlying mechanism of miRNA involvement in salivary gland degeneration remains incompletely understood. Here, we demonstrate that microRNA34-5p (miR-34-5p) regulates the process of salivary gland degeneration in Rhipicephalus haemaphysaloides by modulating the target gene RhECR.MethodsDual luciferase reporter assays and phenotypic rescue experiments identified RhECR as a direct target of miR-34-5p. The overexpression and inhibition of miR-34-5p were quantified by hematoxylin and eosin (H&E) and Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL) staining.ResultsThe results showed that miR-34-5p inhibited the expression of RhECR to retard apoptosis in salivary gland acini. The study identified the roles of miR-34-5p and RhECR and their interactions in tick salivary gland degeneration.ConclusionsThe findings will aid in the application of ECR genes for tick control.

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