Identification of the New GmJAG1 Transcription Factor Binding Motifs Using DAP-Seq

文献类型： 外文期刊

第一作者： Wang, Jinxing

作者： Wang, Jinxing;Zhang, Weiyao;Qu, Mengnan;Gao, Lusi;Pan, Wenjing;Sun, Yanan;Fu, Chunxu;Pu, Zigang;Zhang, Ling;Huang, Mingkun;Hu, Yufang;Pu, Zigang

作者机构：

关键词： Glycine max; GmJAG1; DAP-seq; motifs

期刊名称：PLANTS-BASEL （ 影响因子：4.0； 五年影响因子：4.4 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 12 期

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收录情况： SCI

摘要： Interaction between transcription factors (TFs) and motifs is essential for gene regulation and the subsequent phenotype formation. Soybean (Glycine max) JAGGEED 1 (GmJAG1) is a key TF that controls leaf shape, seed number and flower size. To understand the GmJAG1 binding motifs, in this study, we performed the GmJAG1 DNA affinity purification sequencing (DAP-seq) experiment, which is a powerful tool for the de novo motif prediction method. Two new significant GmJAG1 binding motifs were predicted and the EMSA experiments further verified the ability of GmJAG1 bound to these motifs. The potential binding sites in the downstream gene promoter were identified through motif scanning and a potential regulatory network mediated by GmJAG1 was constructed. These results served as important genomic resources for further understanding the regulatory mechanism of GmJAG1.

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