Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay

文献类型： 外文期刊

第一作者： Liu, Tianqi

作者： Liu, Tianqi;Yang, Bing;Song, Xiaoling;Wang, Xiuhua;Yuan, Yanyan;Liu, Li;Huang, Jie;Liu, Tianqi;Yuan, Yanyan

作者机构：

关键词： Hepatopancreatic parvovirus;Real-time PCR;TaqMan;Detection

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN： 0022-2011

年卷期： 2013 年 114 卷 3 期

页码：

收录情况： SCI

摘要： As one of the major pathogens, hepatopancreatic parvovirus (HPV) can cause severe diseases in penaeid shrimp. We developed a TaqMan-based real-time PCR assay for the HPV detection in China. A pair of primers (HPVF and HPVR) and a TaqMan probe were designed according to the HPV genomic sequence of Chinese isolate (GenBank: GU371276). Our data showed that the primers and TaqMan probe were specific for HPV, and they exhibited no cross-reaction with infectious hypodermal and hematopoietic necrosis virus (IHHNV), white spot syndrome virus (WSSV) and specific pathogen free (SPF) shrimp DNA. The assay had a detection limit of four plasmid HPV DNA copies per reaction. Furthermore, HPV was detected in 16 of 21 Fenneropenaeus Chinensis, 3 of 52 Litopenaeus vannamei and 2 of 2 Marsupenaeus japonicus penaeid shrimp samples. In addition, HPV was also detected in crabs. Therefore, this assay could be successfully used as a sensitive and rapid molecular-based diagnostic method to screen HPV-free animals and survey the prevalence of HPV in cultured populations of penaeid shrimp in China. (C) 2013 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[2]A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Xie Guosi,Huang Jie. 2012

[3]Quantitative detection of the rice false smut pathogen Ustilaginoidea virens by real-time PCR. Li, H.,Ni, D. H.,Li, J.,Li, H.,Ni, D. H.,Duan, Y. B.,Li, J.,Song, F. S.,Li, L.,Wei, P. C.,Yang, J. B.,Li, H.,Wei, P. C.,Duan, Y. B.,Song, F. S.,Chen, Y.. 2013

[4]Detection and quantification of Rhizoctonia cerealis in soil using real-time PCR. Guo, Yingpeng,Li, Wei,Sun, Haiyan,Wang, Ning,Chen, Huaigu,Guo, Yingpeng,Yu, Hanshou. 2012

[5]Development of TaqMan real-time PCR assay for detection and quantitation of reticuloendotheliosis virus. Li, Kai,Gao, Honglei,Gao, Li,Qi, Xiaole,Qin, Liting,Gao, Yulong,Xu, Yanwei,Wang, Xiaomei. 2012

[6]Development of a SYBR Green I real-time PCR for the detection of the orf virus. Wang, Yong,Yang, Kankan,Bai, Caixia,Yin, Dongdong,Qi, Kezong,Wang, Guijun,Li, Yongdong,Li, Gang. 2017

[7]Establishment and application of cross-priming isothermal amplification coupled with lateral flow dipstick (CPA-LFD) for rapid and specific detection of red-spotted grouper nervous necrosis virus. Su, Zi Dan,Shi, Cheng Yin,Huang, Jie,Shen, Gui Ming,Li, Jin,Wang, Sheng Qiang,Fan, Chao,Shi, Cheng Yin,Huang, Jie,Su, Zi Dan,Shen, Gui Ming,Wang, Sheng Qiang,Fan, Chao. 2015

[8]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[9]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[10]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[11]Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Chu, Yue-feng,Cai, Xue-peng,Liu, Xiang-tao,Luo, Xue-nong,Liu, Yong-sheng,Liu, Qing. 2008

[12]Development of cross-priming amplification coupled with vertical flow visualization for rapid detection of infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish, Siniperca chuatsi. Liu, Wenzhi,Zhou, Yong,Fan, Yuding,Jiang, Nan,Zeng, Lingbing,Cain, Kenneth,Cain, Kenneth. 2018

[13]Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification. Liu, Yong-sheng,Liu, Xiang-tao. 2011

[14]The Applications of Gold Nanoparticle-Initialed Chemiluminescence in Biomedical Detection. Liu, Zezhong,Zhao, Furong,Gao, Shandian,Shao, Junjun,Chang, Huiyun. 2016

[15]A Miniature Handheld Automatic Detected Device for Register. Zhang Tiefeng,Wang Jun,Sun Zhihui,Zhao Bo,Lu Guotao. 2011

[16]DEVELOPMENT OF A SIMPLE AND EFFECTIVE METHOD FOR SPECIFIC DETECTION OF PEPPER MILD MOTTLE VIRUS. Liu, Y.,Wang, X.,Zhou, G.,Li, X.. 2009

[17]Aptamer-Based Fluorescence Assay for Hg2+ Determination. Lei Zhao-Jing,Hu Qiu-Hui,Lei Zhao-Jing,Zhang Cun-Zheng,Liu Yuan,Zhang Qiang,Liu Xian-Jin. 2012

[18]Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of ostreid herpesvirus 1 DNA. Ren, Weicheng,Cai, Yuyong,Wang, Chongming,Renault, Tristan,Cai, Yuyong. 2010

[19]Fluorescence polarization assay for the simultaneous determination of bisphenol A, bisphenol F and their diglycidyl ethers in canned tuna. Guan, Tianzhu,Li, Tiezhu,Li, Zhuolin,Wang, Yongzhi,Zhang, Jie,Wang, Yongjun,Zhang, Tiehua,Yu, Hansong,Ruan, Ping. 2017

[20]Validation of high-throughput real time polymerase chain reaction assays for simultaneous detection of invasive citrus pathogens. Loconsole, Giuliana,Savino, Vito,Liao, Hui-Hong,Jiang, Bo,Saponari, Maria,Yokomi, Raymond K.. 2013

作者其他论文 更多>>

微信小程序