Fine mapping of Pa-6 gene for purple apiculus in rice

文献类型： 外文期刊

第一作者： Liu, Xu

作者： Liu, Xu;Sun, Xu;Wang, Wenying;Ding, Hanfeng;Liu, Wei;Yao, Fangyin;Li, Guangxian;Jiang, Mingsong;Zhu, Changxiang

作者机构：

关键词： Pa-6; Physical mapping; Purple apiculus; Rice; Single-segment substitution line (SSSL)

期刊名称：JOURNAL OF PLANT BIOLOGY （ 影响因子：2.434； 五年影响因子：2.455 ）

ISSN： 1226-9239

年卷期： 2012 年 55 卷 3 期

页码：

收录情况： SCI

摘要： Purple apiculus is one of the important agronomic traits of rice. Single-segment substitution line (SSSL) W23-07-6-02-14 in the genetic background of an elite rice variety Huajingxian74 (HJX74) with the substituted interval of RM225-RM217-RM253 on the chromosome 6 was found to have purple apiculus (Pa). To map the gene governing Pa, W23-07-6-02-14 was crossed with the recipient HJX74 to develop an F-2 secondary segregation population. The ratio of purple apiculus to green apiculus showed a good fit to 3:1 ratio, indicating that Pa was controlled by a major dominant gene. The gene locus for Pa was tentatively designated as Pa-6. Using 430 individuals from the F-2 segregation population, the Pa-6 locus was mapped between two SSR markers RM19556 and RM19561 with genetic distances of 0.2 and 0.3 cM, respectively. For fine mapping of the Pa-6 gene, a large F-2:3 segregation population of 3890 individuals was developed from F-2 heterzygous plants in the RM19556-RM19561 region. Recombinant analyses further mapped the Pa-6 gene locus to an interval of 41.7-kb bounded L02 and RM19561. Sequence analysis of this 41.7-kb region revealed that it contains eleven open reading frames (ORFs), of which, ORF5 is classified as the one that is associated with the C (chromogen for anthocyanin) gene, it was presumed to be the candidate gene for Pa. This result provided a foundation of map-based cloning and function analysis of the Pa-6 gene.

分类号： Q94

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