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Molecular cloning, characterization and expression analysis of multiple leptin genes in Jian carp (Cyprinus carpio var. Jian)

文献类型：外文期刊

第一作者： Tang, Yongkai

作者： Tang, Yongkai;Yu, Juhua;Xu, Pao;Tang, Yongkai;Yu, Juhua;Li, Hongxia;Xu, Pao;Li, Jianlin;Ren, Hongtao

作者机构：

关键词： Leptin;Cloning;Expression;Cyprinus carpio var. Jian

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （影响因子：2.231；五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2013 年 166 卷 2 期

页码：

收录情况： SCI

摘要： Three leptin genes (jILEP-A1,jILEP-A2 and jILEP-B) were cloned and characterized in Jian carp (Cyprinus carpi var. jian), with a highly conserved organization encoded by two exons and one intron. jILEP-A1 and jILEP-A2 contained 93 and 102 bp introns, respectively, encoding a 171-amino acid protein. jILEP-B contained a 687 bp intron encoding a 168-aa protein. jILEP-A1 and jILEP-A2 shared 82.5% identity, but only 29.2% and 28.6% with jILEP-B, respectively. jILEP-Al, jILEP-A2, and jILEP-B were expressed in a wide range of tissues, but the expression pattern differed between juveniles and adults (and females and males). In juveniles, the expression of j 1LEP-A1 and jILEPB was higher than jILEP-A2, and was the highest in the liver and gonad. In females, jILEP-A1 and jILEP-A2 expression was the highest in the hypothalamus and liver, whereas jILEP-B mRNA was detected at low levels in all tissues. In males, jILEP-Al mRNA was expressed primarily in the hypothalamus, with only very low levels in the peripheral tissues. jILEP-A2 and j1LEP-B mRNA were primarily expressed in the muscle, hypothalamus, and liver. The expression of j/LEP-Al and jILEP-B mRNA was high in the ovary and testis, respectively. Our results suggest that leptins play an important physiological role in reproduction. (C) 2013 Elsevier Inc All rights reserved.

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