Comprehensive analyses of inhibition of the Chinese traditional food-packaging materials, Indocalamus latifolius leaves (Zongye) on Aspergillus flavus

文献类型： 外文期刊

第一作者： Peng, Wenjing

作者： Peng, Wenjing;Ren, Xiaoxu;Dai, Haiting;Tai, Bowen;Yang, Bolei;Wang, Gang;Li, Xu;Xing, Fuguo

作者机构：

关键词： Indocalamus latifolius; Zongye essential oil; Aspergillus flavus; aflatoxin; inhibitory mechanism

期刊名称：FOOD QUALITY AND SAFETY （ 影响因子：4.4； 五年影响因子：4.8 ）

ISSN： 2399-1399

年卷期： 2024 年 8 卷

页码：

收录情况： SCI

摘要： Objectives The contamination of Aspergillus flavus and aflatoxins (AFs) is one of the most serious safety problems in food and feed. The discovery and application of plant-sourced antifungal agents are hot topics in mycotoxin control. In this study, we aim to develop efficient strategies to control A. flavus and subsequent contamination by AFs.Materials and Methods We focused on Zongye essential oil, which was extracted from Indocalamus latifolius leaves (Zongye, the traditional food-packaging materials). Its antifungal and antitoxin activities were observed by volatile test, and the significant morphological and ultrastructural variations were observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses. The main components of Zongye essential oil were analyzed by gas chromatography-mass spectrometry (GC-MS). RNA sequencing (RNA-Seq) and real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses were used to reveal the inhibitory mechanism.Results Among them, No. 2 Zongye essential oil completely inhibited the growth and toxin production of A. flavus after 10 mu L treatment. Under Zongye essential oil treatment, evidently morphological and ultrastructural variations were observed, such as hyphae shrinkage, partial distortion, and a decrease in conidia number. Longifolene and linalool were the major constituents of Zongye essential oil, accounting for 9.55% and 7.95%, respectively, and linalool had stronger inhibitory effects on fungal growth and mycotoxin biosynthesis than longifolene. Based on the experimental results, the antifungal mechanism was proposed: down-regulations of membrane proteins (AFLA_005560, AFLA_019420, and AFLA_084310, etc.) and conidial genes (fblC, steA, and abaA, etc.) inhibited fungal development, and anti-aflatoxigenic activity might be due to significant down-regulation of AF cluster genes and global regulators (AtfA and AtfB).Conclusion A novel antifungal agent, Zongye essential oil, was identified, and its inhibitory mechanism was comprehensively clarified, which is helpful to control AF contamination in an environmentally friendly way.

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