Mutation of DEFECTIVE EMBRYO SAC1 results in a low seed-setting rate in rice by regulating embryo sac development

文献类型： 外文期刊

第一作者： Hu, Xia

作者： Hu, Xia;Yu, Ping;Zhang, Yingxin;Wu, Weixun;Abbas, Adil;Hong, Yongbo;Sun, Lianping;Liu, Qunen;Xue, Pao;Wang, Beifang;Zhan, Xiaodeng;Cao, Liyong;Cheng, Shihua;Gao, Zhiqiang;Sun, Bin;Deng, Chenwei

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关键词： Embryo sac; female sterile; fertilization; OsDES1; rice; seed-setting rate

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2023 年 74 卷 5 期

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收录情况： SCI

摘要： The seed-setting rate has a significant effect on grain yield in rice (Oryza sativa L.). Embryo sac development is essential for seed setting; however, the molecular mechanism underlying this process remains unclear. Here, we isolated defective embryo sac1 (des1), a rice mutant with a low seed-setting rate. Cytological examination showed degenerated embryo sacs and reduced fertilization capacity in des1. Map-based cloning revealed a nonsense mutation in OsDES1, a gene that encodes a putative nuclear envelope membrane protein (NEMP)-domain-containing protein that is preferentially expressed in pistils. The OsDES1 mutation disrupts the normal formation of functional megaspores, which ultimately results in a degenerated embryo sac in des1. Reciprocal crosses showed that fertilization is abnormal and that the female reproductive organ is defective in des1. OsDES1 interacts with LONELY GUY (LOG), a cytokinin-activating enzyme that acts in the final step of cytokinin synthesis; mutation of LOG led to defective female reproductive organ development. These results demonstrate that OsDES1 functions in determining the rice seed-setting rate by regulating embryo sac development and fertilization. Our study sheds light on the function of NEMP-type proteins in rice reproductive development. OsDES1encodes a NEMP-domain protein that interacts with a cytokinin-activating enzyme, LOG, to determine the seed-setting rate in rice by regulating embryo sac development.

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