Genome Survey of Stipa breviflora Griseb. Using Next-Generation Sequencing

文献类型： 外文期刊

第一作者： Yun, Xiangjun

作者： Yun, Xiangjun;Wu, Jinrui;Sun, Shixian;Liu, Guixiang;Xu, Bo;Zhang, Le;Lv, Shijie;Zhang, Wenguang;Zu, Yazhou;Liu, Bin

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关键词： gene annotation; genome survey; next-generation sequencing; selective sweep analysis; Stipa breviflora Griseb

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.6 ）

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年卷期： 2023 年 13 卷 12 期

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收录情况： SCI

摘要： Due to climate change and global warming, the frequency of sandstorms in northern China is increasing. Stipa breviflora, a dominant species in Eurasian grasslands, can help prevent desertification from becoming more serious. Studies on S. breviflora cover a wide range of fields. To the best of our knowledge, the present study is the first to sequence, assemble, and annotate the S. breviflora genome. In total, 2,781,544 contigs were assembled, and 2,600,873 scaffolds were obtained, resulting in a total length of 649,849,683 bp. The number of scaffolds greater than 1 kb was 70,770. We annotated the assembled genome (>121 kb), conducted a selective sweep analysis, and ultimately succeeded in assembling the Matk gene of S. breviflora. More importantly, our research identified 26 scaffolds that may be responsible for the drought tolerance of S. breviflora Griseb. In summary, the data obtained regarding S. breviflora will be of great significance for future research.

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