Sweet-flavored peptides with biological activities from mulberry seed protein treated by multifrequency countercurrent ultrasonic technology
文献类型: 外文期刊
第一作者: Bian, Yu-Rong
作者: Bian, Yu-Rong;Li, Wen-Jing;Pan, Lian-Han;Peng, Qiang-Min;You, Shuai;Sheng, Sheng;Wang, Jun;Wu, Fu-An;You, Shuai;Sheng, Sheng;Wang, Jun;Wu, Fu-An;Wang, Jun
作者机构:
关键词: Mulberry seed meal protein; Multifrequency countercurrent ultrasonic; treatment; Molecular diffusion dynamics model; Sweet-flavored peptide; Biological activity
期刊名称:FOOD CHEMISTRY ( 影响因子:9.231; 五年影响因子:8.795 )
ISSN: 0308-8146
年卷期: 2022 年 367 卷
页码:
收录情况: SCI
摘要: To increase the reuse of food residues, multifrequency countercurrent ultrasonic treatment was used to enhance the extraction yield of defatted mulberry seed protein (DMSP), and sweet-flavored peptides from DMSP hydrolysates (DMSPHs) were obtained for the first time. Here, the DMSP yield was increased by 16.2% (p < 0.05) while the power density was halved compared with single-frequency ultrasonic treatment. According to Fick's second law, a molecular diffusion dynamics model was developed to be suitable for predicting the pretreatment conditions (R2 = 0.9785). After that, the sweet-flavored peptides were purified and the main amino acid sequences were identified, i.e., FEGGSIE, KDFPEAHSQAT, and GSQPAEGAK. Moreover, the antioxidant activities of DMSPHs prepared with tri-frequency treatment was higher than 60%. The DMSPHs retarded the growth of HepG2 cells in vitro, increased the necrotic quadrant (Q1-UL), and extended the S phase. Therefore, the sweetflavored peptides prepared from DMSPHs using the multifrequency-ultrasonic treatment have significant biological activities.
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