文献类型： 外文期刊

第一作者： Zhang Xiangyue

作者： Zhang Xiangyue;Feng Zili;Zhao Lihong;Liu Shichao;Wei Feng;Shi Yongqiang;Feng Hongjie;Zhu Heqin;Wei Feng;Feng Hongjie;Zhu Heqin

作者机构：

关键词： Cotton; Verticillium wilt; Resistance gene; GhSDH1-1; Salicylic acid

期刊名称：JOURNAL OF COTTON RESEARCH （ 影响因子：2.6； 五年影响因子：2.8 ）

ISSN： 2096-5044

年卷期： 2020 年 3 卷

页码：

收录情况： SCI

摘要： Background: Verticillium wilt, caused by the soil-borne fungus of Verticillium dahliae Kleb., is one of the most devastating diseases of cotton. The complex mechanism underlying cotton resistance to Verticillium wilt remains uncharacterized. Identifying an endogenous resistance gene may be helpful to control this disease. Previous studies revealed that succinate dehydrogenase (SDH) is involved in reactive oxygen species (ROS)-induced stress signaling pathway that is likely to be triggered by salicylic acid (SA). Here, through the metabolomics and differential expression analyses in wilt-inoculated cotton (Gossypium hirsutum), we noticed that GhSDH1-1 gene in cotton may play an important role in the resistance to V. dahlia. Then we reported GhSDH1-1 gene and its functional analysis in relation to the resistance of cotton to V. dahliae. Results: The GhSDH1-1 gene in cotton root was significantly up-regulated after V. dahlia inoculation, and its expression level peaked at 12 and 24 h post-infection. SA can also induce the up-regulation of GhSDH1-1. Additionally, the functional analysis showed that GhSDH1-1-silenced cotton was more susceptible to V. dahliae than the control because of the significant decrease in abundance of immune-related molecules and severe damage to the SA-signaling pathway. In Arabidopsis thaliana, high expression of GhSDH1-1 conferred high resistance to V. dahliae. Arabidopsis that overexpressed GhSDH1-1 had higher resistance to V. dahliae infection compared with the wild-type. Conclusions: Our findings provide new insights into the role of GhSDH1-1; it positively regulates cotton resistance to Verticillium wilt. The regulatory mechanism of GhSDH1-1 is closely related to SA-related signaling pathway.

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