Spectral Analysis of Changes in Photosynthetic Pigment Composition in Leaves of Sweet Cherry Tree Under Rain-Shelter Cultivation Based on Raman and FTIR

文献类型： 外文期刊

第一作者： Zhang Hui-min

作者： Zhang Hui-min;Wen Xiao-peng;Zhang Hui-min;Hou Qian-dong;Tu Kai;Wen Xiao-peng;Wu Ya-wei;Li Quan

作者机构：

关键词： Carotenoids; Chlorophyll; Prunus avium L.; Characteristic peak

期刊名称：SPECTROSCOPY AND SPECTRAL ANALYSIS （ 影响因子：0.589； 五年影响因子：0.504 ）

ISSN： 1000-0593

年卷期： 2021 年 41 卷 4 期

页码：

收录情况： SCI

摘要： To improve the of quality and yield of sweet cherry fruits, sweet cherry trees were cultivated under rain-shelter to avoid the problems of low fruit setting rate, falling fruit and fruit malformation in southern China. Sweet cherry trees under rain-shelter cultivation had an obvious negative effect on photosynthesis. In both plants and algae, photosynthetic pigments such as chlorophylls and carotenoids play irreplaceable roles in light harvesting and mediating stress responses to a variety of endogenous stimuli. This research aimed to detect changes of photosynthetic pigments in leaves that affect photosynthesis of fruit trees quickly and conveniently. The experiment took sweet cherry leaves in two different cultivation pattern, open-field and rain-shelter cultivation, as the research objects, and determined its Raman spectrum in the range of 200 similar to 3500 cm(-1). The analysis is performed, and the characteristic peaks are calibrated and designated from three wave number bands of 400-800, 800 similar to 1250 and 1250 similar to 1650 cm(-1). According to the Raman spectrum characteristic value, it is concluded that the sweet cherry leaves have a relatively small Raman scattering. Sensitivity is mainly concentrated in the 500 similar to 1700 cm(-1) band. The analysis of Raman spectrum in the range of 960 similar to 1800 cm(-1) found that characterizes the carotenoids (lycopene, beta-carotene and lutein) mainly contains 4 main peaks, which are 1526, 1157, 1005 and 960 cm(-1) the Raman intensity of leaves of sweet cherry tree under open-field cultivation is significantly lower than that of rain-shelter cultivation. 1157 and 1526 cm(-1) are also the Raman spectrum characteristic peaks of chlorophyll. Overall analysis shows that photosynthetic pigment content in leaves of sweet cherry under open-field cultivation is lower than that of under rain-shelter cultivation. The characteristic spectral lines of 1 157, 1520 and 1526 cm(-1) correspond to symmetrical stretching vibrations of C-C single bond and C-C double bond, and their

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