Integrating transcriptome and phytohormones analysis provided insights into plant height development in sesame

文献类型： 外文期刊

第一作者： Sheng, Chen

作者： Sheng, Chen;Song, Shengnan;Zhou, Wangyi;Dossou, Senouwa Segla Koffi;Zhou, Rong;Zhang, Yanxin;Li, Donghua;You, Jun;Wang, Linhai;Sheng, Chen

作者机构：

关键词： Sesame; Plant height; RNA-seq; Phytohormones; WGCNA

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2023 年 198 卷

页码：

收录情况： SCI

摘要： Plant height is a key agronomic trait influencing crops yield. The height of sesame plants is important for yield performance, lodging resistance and plant architecture. Although plant height is significantly distinct among sesame varieties, the genetic basis of plant height remains largely unknown. In this study, in order to tackle genetic insights into the sesame plant height development, a comprehensive transcriptome analysis was con-ducted using the stem tips from two sesame varieties with distinct plant height, Zhongzhi13 and ZZM2748, at five time points by BGI MGIseq2000 sequencing platform. A total of 16,952 genes were differentially expressed between Zhongzhi13 and ZZM2748 at five time points. KEGG and MapMan enrichment analyses and quantitative analysis of phytohormones indicated that hormones biosynthesis and signaling pathways were associated with sesame plant height development. Plenty of candidate genes involved in biosynthesis and signaling of brassi-nosteroid (BR), cytokinin (CK) and gibberellin (GA) which were major differential hormones between two va-rieties were identified, suggesting their critical roles in plant height regulation. WGCNA revealed a module which was significantly positively associated with the plant height trait and founded SiSCL9 was the hub gene involved in plant height development in our network. Further overexpression in transgenic Arabidopsis validated the function of SiSCL9 in the increase of plant height by 26.86%. Collectively, these results increase our under-standing of the regulatory network controlling the development of plant height and provide a valuable genetic resource for improvement of plant architecture in sesame.

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