Detection of single nucleotide polymorphism in HTR4 and its relationship with growth traits in sheep

文献类型： 外文期刊

第一作者： Xu, Dan

作者： Xu, Dan;Wang, Xiaojuan;Cheng, Jiangbo;Zhao, Liming;Wang, Jianghui;Lin, Changchun;Yang, Xiaobin;Zhang, Xiaoxue;Wang, Weimin;Zhang, Deyin;Li, Xiaolong;Zhang, Yukun;Zhao, Yuan;Weng, Xiuxiu;Zheng, Wenxin

作者机构：

关键词： HTR4; growth traits; SNP; qRT-PCR; sheep

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：3.7； 五年影响因子：3.3 ）

ISSN： 1049-5398

年卷期： 2023 年

页码：

收录情况： SCI

摘要： In this study, a single nucleotide polymorphism of HTR4 (hydroxytryptamine receptor 4) was detected using DNA sequencing and KASPar (Kompetitive Allele-Specific PCR) technique with the aim of analyzing its effect on growth traits in 1102 sheep. A synonymous mutation g.101220 C> T located on the fifth intron of the ovis HTR4 gene was detected, and association analysis showed that this mutation was significantly associated with growth traits in sheep (p < .05), with TT genotypes having significantly lower body weight, height, length and chest circumference than TC and CC genotypes. It showed that the polymorphism of this locus was significantly associated with growth traits in sheep. In addition, qRT-PCR results showed that HTR4 was expressed in different tissues of sheep. It is highly expressed in the liver, spleen and duodenum. As important metabolic, immune and digestive absorption organs in animals, the above tissues can regulate the excitability of intestinal smooth muscle by participating in the body metabolism and nutrient metabolism of sheep, so that sheep can show better growth characteristics. In conclusion, the polymorphic locus identified in HTR4 gene can be used as candidate molecular marker in sheep breeding.

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