Large-scale bacterial genomic and metagenomic analysis reveals Pseudomonas aeruginosa as potential ancestral source of tigecycline resistance gene cluster tmexCD-toprJ

文献类型： 外文期刊

第一作者： Peng, Kai

作者： Peng, Kai;Sun, Xinran;Wang, Qiaojun;Wang, Zhiqiang;Li, Ruichao;Liu, Yong-Xin;Song, Luyang;Wang, Zhiqiang;Li, Ruichao;Wang, Zhiqiang;Li, Ruichao

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关键词： tmexCD-toprJ; Genemic and metagenomic analysis; Source; Transmission

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：6.7； 五年影响因子：7.1 ）

ISSN： 0944-5013

年卷期： 2024 年 285 卷

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收录情况： SCI

摘要： Background: The global dissemination of the multidrug resistance efflux pump gene cluster tmexCD-toprJ has greatly weakened the effects of multiple antibiotics, including tigecycline. However, the potential origin and transmission mechanisms of the gene cluster remain unclear. Methods: Here, we concluded a comprehensive bioinformatics analysis on integrated 73,498 bacterial genomes, including Pseudomonas spp., Klebsiella spp., Aeromonas spp., Proteus spp., and Citrobacter spp., along with 1,152 long-read metagenomic datasets to trace the origin and propagation of tmexCD-toprJ . Results: Our results demonstrated that tmexCD-toprJ was predominantly found in Pseudomonas aeruginosa sourced from human hosts in Asian countries and North American countries. Phylogenetic and genomic feature analyses showed that tmexCD-toprJ was likely evolved from mexCD-oprJ of some special clones of P. aeruginosa . Furthermore, metagenomic analysis confirmed that P. aeruginosa is the only potential ancestral bacterium for tmexCD-toprJ . A putative mobile genetic structure harboring tmexCD-toprJ , int-int-hp-hp-tnfxB-tmexCD-toprJ , was the predominant genetic context of tmexCD-toprJ across various bacterial genera, suggesting that the two integrase genes play a pivotal role in the horizontal transmission of tmexCD-toprJ . Conclusions: Based on these findings, it is almost certain that the tmexCD-toprJ gene cluster was derived from P. aeruginosa and further spread to other bacteria.

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