Discovery of the dipicolinic acid synthase in the spoilage fungus Paecilomyces reveals its role in spore heat, salt, and alkaline pH resistance

文献类型： 外文期刊

第一作者： Wang, Su-Yan

作者： Wang, Su-Yan;Zhang, Yun-Jiao;Chen, Li-Xiao;Pang, Yi-Bo;Herrera-Balandrano, Daniela D.;Shi, Xin-Chi;Laborda, Pedro;Daly, Paul;Liu, Feng-Quan;Simoes, Marta Filipa;Simoes, Marta Filipa

作者机构：

关键词： Food microbiology; Fungal spores; Environmental resistance; Structural biology; Food spoilage

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.0； 五年影响因子：8.5 ）

ISSN： 0963-9969

年卷期： 2025 年 205 卷

页码：

收录情况： SCI

摘要： Paecilomyces, a food spoilage fungus, releases airborne spores that are highly resistant to harsh environmental conditions, including pasteurization. In this study, dipicolinic acid (DPA), which is a common component of bacterial spores, was detected in Paecilomyces maximus conidia at concentrations from 0.1 % to 2.8 % w/w (DPA weight per spore weight). The DPA synthase (PmDpa) was identified and obtained through heterologous expression in Escherichia coli. The enzyme was iron-dependent and catalyzed the conversion of L-2,3-dihydrodipicolinate (DHD) into DPA in the presence of NAD+. Mutant Delta pmdpa spores exhibited altered morphology and reduced resistance to heat, salinity, and alkaline pH compared to wild-type spores, indicating that PmDpa plays a key role in the structure and environmental tolerance of P. maximus spores. DPA was identified in the spores of several Paecilomyces, Aspergillus, and Penicillium strains, indicating its prevalence in the conidia of Thermoascaceae and Aspergillaceae (Eurotiales). This study identifies DPA in Eurotiales spores for the first time and highlights the first key gene associated with Paecilomyces spore resistance.

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