Identification of a major QTL and candidate genes analysis for branch angle in rapeseed (Brassica napus L.) using QTL-seq and RNA-seq

文献类型： 外文期刊

第一作者： Lei, Shaolin

作者： Lei, Shaolin;Liang, Fenghao;Zhang, Yuling;Zhang, Chao;Xiao, Huagui;Tang, Rong;Yang, Bin;Wang, Lulu;Jiang, Huanhuan;Chen, Li

作者机构：

关键词： rapeseed; branch angle; BSA-seq; QTL mapping; RNA-Seq

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2024 年 15 卷

页码：

收录情况： SCI

摘要： Introduction: Branching angle is an essential trait in determining the planting density of rapeseed (Brassica napus L.) and hence the yield per unit area. However, the mechanism of branching angle formation in rapeseed is not well understood. Methods: In this study, two rapeseed germplasm with extreme branching angles were used to construct an F-2 segregating population; then bulked segregant analysis sequencing (BSA-seq) and quantitative trait loci (QTL) mapping were utilized to localize branching anglerelated loci and combined with transcriptome sequencing (RNA-seq) and quantitative real-time PCR (qPCR) for candidate gene mining Results and discussion: A branching angle-associated quantitative trait loci (QTL) was mapped on chromosome C3 (C3: 1.54-2.65 Mb) by combining BSA-seq as well as traditional QTL mapping. A total of 54 genes had SNP/Indel variants within the QTL interval were identified. Further, RNA-seq of the two parents revealed that 12 of the 54 genes were differentially expressed between the two parents. Finally, we further validated the differentially expressed genes using qPCR and found that six of them presented consistent differential expression in all small branching angle samples and large branching angles, and thus were considered as candidate genes related to branching angles in rapeseed. Our results introduce new candidate genes for the regulation of branching angle formation in rapeseed, and provide an important reference for the subsequent exploration of its formation mechanism.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Linkage Mapping and Discovery of Candidate Genes for Drought Tolerance in Rice During the Vegetative Growth Period

    作者：Jiao, Aixia;Jiao, Aixia;Ma, Xiaoding;Cui, Di;Han, Bing;Han, Longzhi;Ma, Xiaoding;Cui, Di;Han, Bing;Han, Longzhi;Chen, Li;Ma, Jing;Sun, Jianchang

    关键词：Rice; Drought tolerance; QTL; Candidate gene; Haplotype

  • Overexpression of AcWRKY31 Increases Sensitivity to Salt and Drought and Improves Tolerance to Mealybugs in Pineapple

    作者：Wai, Myat Hnin;Luo, Tiantian;Zhou, Qiao;Mohammadi, Mohammad Aqa;Cheng, Han;Aslam, Mohammad;Liu, Chang;Chai, Gaifeng;Huang, Dongping;Liu, Yanhui;Cai, Hanyang;Wang, Xiaomei;Qin, Yuan;Wang, Lulu;Wai, Myat Hnin;Luo, Tiantian;Zhou, Qiao;Mohammadi, Mohammad Aqa;Cheng, Han;Aslam, Mohammad;Liu, Chang;Chai, Gaifeng;Huang, Dongping;Liu, Yanhui;Cai, Hanyang;Wang, Xiaomei;Qin, Yuan;Wang, Lulu;Wai, Myat Hnin;Luo, Tiantian;Zhou, Qiao;Mohammadi, Mohammad Aqa;Cheng, Han;Aslam, Mohammad;Liu, Chang;Chai, Gaifeng;Huang, Dongping;Liu, Yanhui;Cai, Hanyang;Wang, Xiaomei;Qin, Yuan;Wang, Lulu;Wai, Myat Hnin;Priyadarshani, S. V. G. N.;Wang, Xiaomei

    关键词：pineapple; WRKY transcription factor; AcWRKY31; biotic stress; abiotic stress

  • Effects of plant tissue permeability on invasion and population bottlenecks of a phytopathogen

    作者：Jiang, Gaofei;Shi, Xiaojun;Chen, Xinping;Ding, Wei;Zhang, Yong;Jiang, Gaofei;Zhang, Yuling;Jousset, Alexandre;Zhao, Fang-Jie;Xu, Yangchun;Shen, Qirong;Wei, Zhong;Chen, Min;Zhang, Yong;Ramoneda, Josep;Han, Liangliang;Shi, Yu;Peyraud, Remi;Wang, Yikui;Hikichi, Yasufumi;Ohnishi, Kouhei;Dini-Andreote, Francisco;Dini-Andreote, Francisco;Dini-Andreote, Francisco

    关键词：

  • Changes in physicochemical properties and formation process of colloidal nanoparticles (CNPs) during the lamb soup stewing

    作者：Fu, Jianing;Li, Shaobo;Xu, Meizhen;Zhang, Dequan;Chen, Li;Fu, Jianing

    关键词：Colloidal nanoparticles; Lamb soup; Structure; Chemical localization; Formation mechanism

  • Recent progress in perylene diimide supermolecule-based photocatalysts

    作者：Yang, Bin;Lu, Liliang;Liu, Shiyu;Cheng, Wenjin;Liu, Hao;Huang, Chao;Meng, Xintao;Jia, Xin;Meng, Xintao;Rodriguez, Raul D.

    关键词：

  • Development of an analytic method for organosulfur compounds in Welsh onion and its use for nutritional quality analysis of five typical varieties in China

    作者：Liu, Pingxiang;Bi, Jingxiu;Jiang, Yuying;Gao, Rui;Gao, Lei;Zhao, Tong;Zhang, Xiao;Zhang, Chao;Wang, Yutao;Wu, Peng;Li, Yonghua

    关键词：Enzymatic inhibition; UHPLC-MS/MS; Organosulfur compound; Amino acid; Nutritional quality

  • Fungal diversity notes 1717-1817: taxonomic and phylogenetic contributions on genera and species of fungal taxa

    作者：Liu, Shi-Liang;Wang, Xue-Wei;Razaghi, Parisa;Raza, Mubashar;Cai, Lei;Jiang, Shuhua;Zhang, Chao;Zhou, Li-Wei;Wang, Xue-Wei;Li, Guo-Jie;Deng, Chun-Ying;Rossi, Walter;Leonardi, Marco;Liimatainen, Kare;Niskanen, Tuula;Kekki, Tapio;Smith, Matthew E.;Ammirati, Joe;Ammirati, Joe;Bojantchev, Dimitar;Abdel-Wahab, Mohamed A.;Zhang, Ming;Tian, Enjing;Bau, Tolgor;Lu, Yong-Zhong;Zhang, Jing-Yi;Ma, Jian;Zhang, Jing-Yi;Ma, Jian;Du, Tian-Ye;Peng, Xing-Can;Hyde, Kevin D.;Dutta, Arun Kumar;Acharya, Krishnendu;Saha, Rituparna;Tarafder, Entaj;Du, Tian-Ye;Du, Tian-Ye;Peng, Xing-Can;Hyde, Kevin D.;Xu, Jize;Kim, Ji Seon;Lim, Young Woon;Kim, Ji Seon;Lim, Young Woon;Gerlach, Alice;Zeng, Nian-Kai;Han, Yun-Xiao;Raza, Mubashar;Calabon, Mark S.;Jones, E. B. Gareth;Kumar, T. K. Arun;Krishnapriya, K.;Thomas, Anjitha;Kaliyaperumal, Malarvizhi;Kezo, Kezhocuyi;Gunaseelan, Sugantha;Singh, Sanjay Kumar;Singh, Paras Nath;Lagashetti, Ajay Chandrakant;Pawar, Kadambari Subhash;Rana, Shiwali;Zhang, Chao;Zhang, Huang;Qing, Yun;Peng, Xing-Can;Wen, Ting-Chi;Peng, Xing-Can;Wen, Ting-Chi;Ramirez, Natalia A.;Niveiro, Nicolas;Li, Mei-Xiang;Yang, Zhu L.;Wu, Gang;Li, Mei-Xiang;Yang, Zhu L.;Wu, Gang;Tennakoon, Danushka S.;Suwannarach, Nakarin;Kumla, Jaturong;Lumyong, Saisamorn;Tennakoon, Danushka S.;Suwannarach, Nakarin;Kumla, Jaturong;Lumyong, Saisamorn;Kuo, Chang-Hsin;da Silva, Tatiane M.;Bezerra, Jadson D. P.;Souza-Motta, Cristina M.;He, Gang;Ji, Xiao-Hong;Wannathes, Nopparat

    关键词：95 new taxa; Six new records; Ascomycota; Basidiomycota

微信小程序