Genetic characterization of duck hepatitis B viruses from Anhui Province, China

文献类型： 外文期刊

第一作者： Ye, Yumeng

作者： Ye, Yumeng;Li, Hang;Liu, Yuhan;Meng, Chunchun;Zhu, Jie;Liu, Guangqing;Li, Chuanfeng;Ye, Yumeng;Wang, Yong;Liu, Yuhan

作者机构：

关键词： Duck hepatitis B virus; Hepadnavirus; Alignment; Phylogenetic analysis; Recombination

期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.2； 五年影响因子：2.8 ）

ISSN： 1517-8382

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Duck hepatitis B virus (DHBV) infection model was frequently used as the experimental model for human hepatitis B virus (HBV) research. In order to decipher the genetic characteristics of DHBVs from Anhui province of China, 120 duck liver tissue samples were collected and subjected to PCR screening, and 28 samples were detected as DHBV positive. Subsequently, five DHBV-positive samples were selected for genome-wide amplification and a comprehensive analysis. Comparative analysis of complete genome sequences using the MegAlign program showed that five strains of DHBVs shared 94.5-96.3% with each other and 93.2-98.7% with other reference strains in GenBank. The phylogenetic analysis showed that all five DHBV strains belonged to the evolutionary branch of "Chinese DHBV" isolates or DHBV-2. Importantly, three potential intra-genotypic recombination events, between strains AAU-6 and Guilin, strains AAU-1 and GD3, and strains AAU-6 and AAU-1, were respectively found using the RDP and SimPlot softwares and considered the first report in avihepadnaviruses. These results not only improve our understanding for molecular prevalence status of DHBV among ducks, but also provide a reference for recombination mechanism of HBV.

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