Enrichment methods of N-linked glycopeptides from human serum or plasma: A mini-review

文献类型： 外文期刊

第一作者： Chao, Xuyuan

作者： Chao, Xuyuan;Zhang, Baoying;Yang, Shengjie;Zhang, Jingyi;Zang, Xin;Chen, Lu;Qi, Lu;Wang, Xinghe;Liu, Xizi;Hu, Han

作者机构：

关键词： Human serum or plasma; Human blood; Carbohydrate; N-linked glycopeptides; Enrichment methods; Cancer; Glycoproteomics

期刊名称：CARBOHYDRATE RESEARCH （ 影响因子：3.1； 五年影响因子：2.5 ）

ISSN： 0008-6215

年卷期： 2024 年 538 卷

页码：

收录情况： SCI

摘要： Human diseases often correlate with changes in protein glycosylation, which can be observed in serum or plasma samples. N-glycosylation, the most common form, can provide potential biomarkers for disease prognosis and diagnosis. However, glycoproteins constitute a relatively small proportion of the total proteins in human serum and plasma compared to the non-glycosylated protein albumin, which constitutes the majority. The detection of microheterogeneity and low glycan abundance presents a challenge. Mass spectrometry facilitates glycoproteomics research, yet it faces challenges due to interference from abundant plasma proteins. Therefore, methods have emerged to enrich N-glycans and N-linked glycopeptides using glycan affinity, chemical properties, stationary phase chemical coupling, bioorthogonal techniques, and other alternatives. This review focuses on N-glycans and N-glycopeptides enrichment in human serum or plasma, emphasizing methods and applications. Although not exhaustive, it aims to elucidate principles and showcase the utility and limitations of glycoproteome characterization.

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