Molecular characterization of five steroid receptors from pengze crucian carp and their expression profiles of juveniles in response to 17 alpha-ethinylestradiol and 17 alpha-methyltestosterone

文献类型： 外文期刊

第一作者： Zheng, Yao

作者： Zheng, Yao;Li, Meng;Qin, Fang;Liu, Shaozhen;Wang, Houpeng;Wu, Tingting;Zhang, Yingying;Wang, Zaizhao;Wang, Lihong;Liang, Hongwei

作者机构：

关键词： 17 alpha-Ethinylestradiol;17 alpha-Methyltestosterone;Juvenile pengze crucian carp;Estrogen receptor;Androgen receptor;Vitellogenin

期刊名称：GENERAL AND COMPARATIVE ENDOCRINOLOGY （ 影响因子：2.822； 五年影响因子：2.772 ）

ISSN： 0016-6480

年卷期： 2013 年 191 卷

页码：

收录情况： SCI

摘要： Pengze crucian carp (Carassius auratus var. pengze, Pcc), a triploid gynogenetic fish, was used in this study to investigate the cross-talk between EDCs and steroid receptors. The full-length cDNAs of five steroid receptors (esr1, er alpha2, esr2a, esr2b, ar) and partial cDNA of vtg B were isolated. The tissue distributions of these genes were analyzed in adult fish by qRT-PCR. Then the expression profiles of five steroid receptors (esrs and ar) and vtg B were detected in the juveniles exposed to 17 alpha-ethinylestradiol (EE2, 0.1, 1 and 10 ng/L) and 17 alpha-methyltestosterone (MT, 50 mu g/L) for 4 weeks. The results demonstrated that esrs, ar, and vtg B were predominantly expressed in liver of adult fish. However, among these detected genes, esr1 and er alpha2 mRNAs are sensitive biomarkers in response to EE2 at 0.1, 1, and 10 ng/L for 1 and 2 weeks compared to esr2a, esr2b, ar, and vtg B in the juveniles of mono-female gynogenetic fish. Totally, the subtypes of esrs show biphasic responses to EE2 exposures for 4 weeks, and most of the EE2 exposures at 0.1, 1, and 10 ng/L for 1, 2, 3 and 4 weeks did not induce the mRNA expressions of vtg B. However, 1-, 2-, and 4-week 50 mu g/L MT all significantly stimulated vtg B transcripts. Further investigations are needed to elucidate the mechanism underlying the insensitivity or down-regulation of vtg B mRNA in response to EE2 in juvenile Pcc. (C) 2013 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells. Zhang, H.,Chen, F.,Tao, Z. R.,Li, J. J.,Lu, L. Z.,Zhang, H.,Chen, F.,Li, G. L.,Ding, Y. Y.,Zhong, S. L.. 2014

[2]Delayed and persistent ERK1/2 activation is required for 4-hydroxytamoxifen-induced cell death. Zhou, Jian-Hua,Yu, David V.,Cheng, Jingwei,Shapiro, David J.. 2007

[3]Effect of 17 alpha-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus. Zheng, Yao,Qu, Jianhong,Qiu, Liping,Fan, Limin,Meng, Shunlong,Song, Chao,Bing, Xuwen,Chen, Jiazhang,Zheng, Yao,Qu, Jianhong,Qiu, Liping,Fan, Limin,Meng, Shunlong,Song, Chao,Bing, Xuwen,Chen, Jiazhang,Zheng, Yao,Bing, Xuwen,Chen, Jiazhang. 2016

[4]Molecular cloning of Pcc-dmrt1s and their specific expression patterns in Pengze crucian carp (Carassius auratus var. Pengze) affected by 17 alpha-methyltestosterone. Zheng, Yao,Xu, Peng,Li, Meng,Wang, Zaizhao,Liang, Hongwei. 2014

[5]Molecular mechanism of endocrine system impairment by 17 alpha-methyltestosterone in gynogenic Pengze crucian carp offspring. Zheng, Yao,Liu, Yan,Gao, Jiancao,Yang, Yanping,Zhang, Yingying,Wang, Zaizhao,Zheng, Yao,Chen, Jiazhang,Yang, Yanping,Bing, Xuwen,Zheng, Yao,Chen, Jiazhang,Yang, Yanping,Bing, Xuwen,Zheng, Yao,Chen, Jiazhang,Yang, Yanping,Bing, Xuwen,Zheng, Yao,Gao, Zexia,Liang, Hongwei.

[6]Vitellogenin and vitellogenin receptor gene expression profiles in Spodoptera exigua are related to host plant suitability. Zhao, Jing,Sun, Yang,Xiao, Liubin,Tan, Yongan,Jiang, Yiping,Bai, Lixin,Zhao, Jing. 2018

[7]Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages. Liao, T.,Guo, Q. L.,Xu, Y.,Liao, T.,Cheng, W.,Jin, S. W.. 2009

[8]Characterization of a pattern recognition molecule vitellogenin from carp (Cyprinus carpio). Liu, Qing-Hui,Gao, Chun-Ren,Zhang, Shi-Cui,Li, Zhao-Jie. 2009

[9]Molecular components related to egg quality during the reproductive season of turbot (Scophthalmus maximus). Jia, Yudong,Meng, Zhen,Liu, Xinfu,Lei, Jilin.

[10]Molecular cloning, expression, purification and characterization of vitellogenin in scallop Patinopecten yessoensis with special emphasis on its antibacterial activity. Wu, Biao,Ji, Guangdong,Wu, Biao,Liu, Zhihong,Zhou, Liqing,Yang, Aiguo.

[11]Effect of dietary n-3 LC-PUFAs on plasma vitellogenin, sex steroids, and ovarian steroidogenesis during vitellogenesis in female silver pomfret (Pampus argenteus) broodstock. Peng, Shiming,Gao, Quanxin,Shi, Zhaohong,Zhang, Chenjie,Wang, Jiangang,Yin, Fei,Zhang, Yanliang.

[12]Chrysopa septempunctata (Neuroptera: Chrysopidae) Vitellogenin Functions Through Effects on Egg Production and Hatching. Liu, C.,Mao, J.,Zeng, F.,Liu, C..

[13]Enhanced effects by mixtures of three estrogenic compounds at environmentally relevant levels on development of Chinese rare minnow (Gobiocypris rarus). Yang, Fangxing,Jin, Shiwei,Jin, Shiwei,Hui, Yang,Xu, Ying,Liao, Tao,Wen, Sheng.

[14]Dietary betaine activates hepatic VTGII expression in laying hens associated with hypomethylation of GR gene promoter and enhanced GR expression. Omer, Nagmeldin A.,Hu, Yun,Idriss, Abdulrahman A.,Abobaker, Halima,Hou, Zhen,Dong, Haibo,Zhao, Ruqian,Omer, Nagmeldin A.,Hu, Yun,Idriss, Abdulrahman A.,Abobaker, Halima,Hou, Zhen,Dong, Haibo,Zhao, Ruqian,Zhao, Ruqian,Hu, Yan. 2018

[15]Sublethal effects of sulfoxaflor on biological characteristics and vitellogenin gene (AlVg) expression in the mirid bug, Apolygus lucorum (Meyer-Dur). Zhen, Congai,Miao, Ling,Gao, Xiwu,Zhen, Congai. 2018

[16]Tradeoff between reproduction and resistance evolution to Bt-toxin in Helicoverpa armigera: regulated by vitellogenin gene expression. Xiao, H. J.,Liang, G. M.,Guo, Y. Y.,Wu, K. M.,Xiao, H. J.. 2014

[17]Molecular characterisation of the vitellogenin gene (AlVg) and its expression after Apolygus lucorum had fed on different hosts. Xiao, Liubin,Xu, Guangchun,Zhao, Jing,Tan, Yongan,Bai, Lixin,Sun, Yang,Cao, Guangchun,Zhang, Yongjun,Xiao, Yingfang.

[18]Molecular Cloning and the Expression Profile of Vitellogenin in Relation to Tissue and Food Source in Apolygus lucorum (Hemiptera: Miridae). Yuan, Wei,Zhao, Xincheng,Wu, Kongming.

[19]Dynamics of Persistent and Acute Deformed Wing Virus Infections in Honey Bees, Apis mellifera. Evans, Jay D.,Hamilton, Michele,Chen, Yan Ping,Di Prisco, Gennaro,Pennacchio, Francesco,Caprio, Emilio,Zhang, Xuan,Li, Jilian,DeGrandi-Hoffman, Gloria. 2011

[20]The nuclear hormone receptor E75A regulates vitellogenin gene (Al-Vg) expression in the mirid bug Apolygus lucorum. Tan, Y. -A.,Zhao, X. -D.,Hao, D. -J.,Sun, Y.,Zhao, J.,Jiang, Y. -P.,Bai, L. -X.,Xiao, L. -B.. 2018

作者其他论文 更多>>

微信小程序