Integrated full-length transcriptome and metabolome analysis reveals the defence response of melon to gummy stem blight

文献类型： 外文期刊

第一作者： Wang, Haiyan

作者： Wang, Haiyan;Wei, Xiaoying;Mo, Changjuan;Wei, Minghua;Li, Yaqiong;Fan, Yuxin;Gu, Xiaojing;Kong, Qiusheng;Zhang, Xuejun;Zhang, Yongbing

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关键词： alternative splicing; differentially accumulated metabolites; differentially expressed genes; novel genes; plant-pathogen interaction

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.3； 五年影响因子：8.1 ）

ISSN： 0140-7791

年卷期： 2024 年 47 卷 6 期

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收录情况： SCI

摘要： Gummy stem blight (GSB), a widespread disease causing great loss to cucurbit production, has become a major threat to melon cultivation. However, the melon-GSB interaction remains largely unknown. Here, full-length transcriptome and widely targeted metabolome were used to investigate the defence responses of resistant (PI511089) and susceptible (Payzawat) melon accessions to GSB pathogen infection at 24 h. The biosynthesis of secondary metabolites and MAPK signalling pathway were specifically enriched for differentially expressed genes in PI511890, while carbohydrate metabolism and amino acid metabolism were specifically enriched in Payzawat. More than 1000 novel genes were identified and MAPK signalling pathway was specifically enriched for them in PI511890. There were 11 793 alternative splicing events involving in the defence response to GSB. Totally, 910 metabolites were identified in Payzawat and PI511890, and flavonoids were the dominant metabolites. Integrated full-length transcriptome and metabolome analysis showed eriodictyol and oxalic acid were the potential marker metabolites for GSB resistance in melon. Moreover, posttranscription regulation was widely involved in the defence response of melon to GSB pathogen infection. These results not only improve our understanding on the interaction between melon and GSB, but also facilitate the genetic improvement of melon with GSB resistance. The early defence responses of resistant and susceptible melon (Cucumis melo L.) accessions to gummy stem blight infection caused by Stagonosporopsis cucurbitacearum were investigated and compared using the full-length transcriptome, widely targeted metabolome and histochemical staining methods.

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