Mining, Identification, and Characterization of Three Xylanases from the Microbiota of T. fuciformis with Its Companion Strains

文献类型： 外文期刊

第一作者： Lin, Yanhuan

作者： Lin, Yanhuan;Li, Changle;Wei, Chenxin;Zhang, Liaoyuan;Lin, Hui

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关键词： xylanase; T. fuciformis; companion strain; metagenome; heterologous expression

期刊名称：CATALYSTS （ 影响因子：3.9； 五年影响因子：4.2 ）

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年卷期： 2024 年 14 卷 1 期

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收录情况： SCI

摘要： Microbial xylanase has wide application in bioenergy, animal feed, environmental protection, the pulp and paper industry, and agricultural development. In this study, three xylanases from the microbiota of T. fuciformis with its companion strains were identified by metagenomics sequencing. The three enzymes were subjected to cloning and expression in E. coli or P. pastoris, purification, and characterization for their properties. The results showed that AsXyn1, from Annulohypoxylon stygium, among the three enzymes possessed high thermostability at 40 degrees C and broad pH tolerance in the range of 2.0-10.0, exhibiting its application potential. Furthermore, it was found that post-translational modification (such as glycosylation) of AsXyn1 enzyme modulated its activity, kinetic parameters, and thermostability. These results and findings provided a hint for enzyme modification and design in future.

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