The First Genomic Analysis of Visna/Maedi Virus Isolates in China

文献类型： 外文期刊

第一作者： Wu, Jian-Yong

作者： Wu, Jian-Yong;Mi, Xiao-Yun;Yang, Xue-Yun;Wei, Jie;Meng, Xiao-Xiao;Bolati, Hongduzi;Wei, Yu-Rong

作者机构：

关键词： Maedi-Visna virus; provirus; Illumina sequencing; cloning; phylogenetic analysis

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.471； 五年影响因子：3.821 ）

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年卷期： 2022 年 9 卷

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收录情况： SCI

摘要： Visna/Maedi virus (VMV) is a neglected pathogen that damages sheep and goats' nervous and respiratory systems. The virus was discovered 80 years ago and has been endemic in China for nearly four decades; nevertheless, there is little information regarding Chinese isolates' genotypes and genomic characteristics. In this study, the proviral DNA of strains isolated in 1985 and 1994 were extracted, and the proviral DNA was subjected to Illumina sequencing combined with Sanger sequencing of poor coverage regions. The results showed that the two isolates were clustered with genotype A2 and shared 78.3%-89.1% similarity to reference VMV genome sequences, with the highest similarity (88.7%-89.1%) to the USA strain USMARC-200212120-r (accession no. ) and lowest similarity (78.3%-78.5%) to the Italian strain SRLV009 (accession no. ). A maximum-likelihood tree showed that the Chinese VMV strains and the USA strain 1150 (accession no. ) comprise a monophyletic group with a short tree branch. Our data filled the gap in genomic analysis and viral evolution in Chinese VMV strains, and would be benefit China's source-tracing and eradication program development in China.

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