Intrasporangium zincisolvens sp. nov., A Novel Actinobacterium Isolated from Rhizosphere Soil

文献类型： 外文期刊

第一作者： Feng, Lu Yao

作者： Feng, Lu Yao;Shi, Zhu Feng;Pu, Te;Shi, Zhu Li;Liu, Xin;Wang, Yu;Yang, Pei-Wen;Feng, Lu Yao;Zhao, Jiang Yuan;Li, Ming Gang;Shi, Zhu Li;Liu, Xin;Wang, Yu;Li, Le Le

作者机构：

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.6； 五年影响因子：2.6 ）

ISSN： 0343-8651

年卷期： 2025 年 82 卷 2 期

页码：

收录情况： SCI

摘要： A novel phosphate-solubilizing and zinc-solubilizing actinobacterium strain YIM S08009T was isolated from rhizosphere soil collected from Pinus yunnanensis in Wuliangshan National Nature Reserve, Pu'er City, Yunnan Province, southwest PR China. Cells of strain YIM S08009T were Gram-stain-positive, non-motile, irregular rods to cocci, and formed yellow and white colonies on nutrient agar. Growth was observed at 10-40 degrees C (optimum 25-35 degrees C), pH 6.0-8.5 (optimum 7), and 0-4% (w/v) NaCl (optimum 1%). The cell wall peptidoglycan contained LL-diaminopimelic acid. The whole-cell sugars were mannose, ribose, glucose, and galactose. The predominant menaquinone was MK-8(H4). Major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, unknown lipid, and 3 unknown phospholipids. The predominant fatty acids were iso-C14:0, iso-C15:0, and iso-C16:0. The DNA G + C content was 72.6%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM S08009T belonged to genus Intrasporangium, and was most closely related to Intrasporangium flavum MUSC 78T, with 99.0% 16S rRNA gene sequence similarity. Strain YIM S08009T shared 90.1% orthologous average nucleotide identity (OrthoANI) and 39.8% digital DNA-DNA hybridization (dDDH) with I. flavum MUSC 78T. The genome of strain YIM S08009T contained phosphate-solubilizing genes (SenX3, RegX3, pstSCAB, ugpBAEC, phoA) and zinc-solubilizing genes (znuABC, zupT), and the strain had also demonstrated in vitro phosphorus and zinc solubilization. Based on the genotypic and phenotypic analyses, strain YIM S08009T (= CGMCC 1.60168T = NBRC 116604T = KCTC 59021T) represents a novel Intrasporangium species, for which the name Intrasporangium zincisolvens sp. nov. is proposed.

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