Cloning, characterization and expression of a cDNA encoding a granulin-like polypeptide in Ciona savignyi
文献类型: 外文期刊
第一作者: Zhao, Jin
作者: Zhao, Jin;Liu, Ming;Xiao, Lin;Wu, Ning;Liu, Ge;Lin, Xiukun;Zhao, Jin;Xiao, Lin;Liu, Ge;Wei, Jianteng;Huang, Haijuan;Zhang, Yuyan;Zheng, Lanhong;Zheng, Lanhong;Lin, Xiukun
作者机构:
关键词: Ciona savignyi;Granulin;Antitumor polypeptide;Apoptosis
期刊名称:BIOCHIMIE ( 影响因子:4.079; 五年影响因子:4.104 )
ISSN: 0300-9084
年卷期: 2013 年 95 卷 8 期
页码:
收录情况: SCI
摘要: Previous study in our laboratory confirmed that a novel polypeptide, CS5931 derived from Ciona savignyi possesses potent antitumor activity. In the present study, the full length cDNA of CS5931 precursor, termed Cs-pgrn-1 was cloned. The complete cDNA sequence of this gene consists of 685 bp containing an open reading frame (ORF) of 522 bp (173 amino acid residues). In silico analysis revealed that the polypeptide consists of two identical domains, similar with granulin (GRN) found in other species, and each of the domain encodes a polypeptide identical with CS5931. Phylogenetic analysis confirmed that CS5931 shares high homology with Ciona intestinalis GRN and is conserved during evolution. The polypeptide also shows high similarity with human GRN A, B, and C. Prediction of 3D protein structure revealed the 3D structure of CS5931 is very similar with human GRN A. The CS5931 was expressed using a prokaryotic expression system and the purified polypeptide inhibited the growth of several tumor cell lines in vitro via apoptotic pathway. Our study revealed that CS5931 has the potential to be developed as a novel antitumor agent. (C) 2013 Elsevier Masson SAS. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Antioxidant activity of Phellinus igniarius fermentation mycelia contributions of different solvent extractions and their inhibitory effect on α-amylase
作者:Dong, Yating;Wang, Tao;Gan, Bingcheng;Zhao, Jin;Duan, Xinlian;Cao, Luping;Feng, Rencai;Miao, Renyun;Yan, Junjie;Wu, Zhi;Dong, Yating;Wang, Tao;Gan, Bingcheng;Zhao, Jin;Duan, Xinlian;Cao, Luping;Feng, Rencai;Miao, Renyun;Yan, Junjie;Wu, Zhi;Wasser, Solomon P.;Wasser, Solomon P.;Zhang, Zhiyuan
关键词:Phellinus igniarius QB72; Fermentation mycelia; Polysaccharides; alpha-amylase inhibitory
-
Exploring the Influence of Culture Environment on the Yield of Volvariella volvacea Based on Microbiomics
作者:Liu, Zhu;Liu, Ming;Liu, Zhu;Wang, Jianhao;Peng, Yangyang;Zhou, Hui;Liu, Ming;Liu, Zhu;Ye, Luyao;Kang, Linzhi
关键词:Volvariella volvacea; edible mushroom; metagenome; microbial community
-
Dynamic behaviors of protein and water associated with fresh noodle quality during processing based on different HMW-GSs at Glu- D1
作者:Zhang, Yuyan;Zhou, Hongwei;Guo, Boli;Zhang, Yingquan;Guo, Boli;Zhang, Yingquan;Zhang, Yuyan;Zhao, Haiyan;Zhou, Hongwei;Zhang, Xiaoke
关键词:Fresh noodle; HMW-GSs; Near-isogenic lines; Gluten network; Water migration
-
Single-molecule assay guided crRNA optimization enhances specific microRNA detection by CRISPR-Cas12a
作者:Chen, Kaizhao;Zhong, Mingtian;Xie, Jiaqi;Huo, Yongkun;Ma, Xiaodong;Chen, Kaizhao;Zhong, Mingtian;Xie, Jiaqi;Huo, Yongkun;Ma, Xiaodong;Sun, Wenjun;Lu, Xiuqi;Chen, Ziting;Sun, Bo;Huang, Xingxu;Huang, Xingxu;Wang, Xinjie;Liu, Ming;Ma, Peixiang
关键词:MicroRNA; CRISPR; Cas12a; Stem-loop; Detection
-
Improved photosynthetic performance under unilateral weak light conditions in a wide-narrow-row intercropping system is associated with altered sugar transport
作者:Chen, Guopeng;Zhao, Xuyang;Bawa, George;Liang, Bing;Feng, Liang;Pu, Tian;Yong, Taiwen;Liu, Weiguo;Liu, Jiang;Du, Junbo;Yang, Feng;Wu, Yushan;Liu, Chunyan;Wang, Xiaochun;Yang, Wenyu;Chen, Guopeng;Zhao, Xuyang;Bawa, George;Liang, Bing;Feng, Liang;Pu, Tian;Yong, Taiwen;Liu, Weiguo;Liu, Jiang;Du, Junbo;Yang, Feng;Wu, Yushan;Liu, Chunyan;Wang, Xiaochun;Yang, Wenyu;Liu, Ming
关键词:Intercropping; light; maize; photosynthesis; sugar allocation; sugar metabolism
-
Production of cleavage-resistant phytase transgenic pigs by handmade cloning
作者:Zhang, Mingyuan;Zhang, Mingyuan;Yang, Haowen;Chen, Shijin;Chen, Xiaoliang;Huang, Yuemeng;Chen, Baojian;Wu, Yanjun;Jiang, Qinyang;Guo, Yafen;Wei, Yingming;Lan, Ganqiu;Liang, Jing;Jiang, Hesheng;Zhang, Mingyuan;Zhang, Jun;Guo, Xiaoping;Yang, Haowen;Chen, Xiaoliang;Lin, Xiukun
关键词:Cleavage-resistant phytase; Handmade cloning; Transgenic pigs
-
Comparative analysis on root exudate and rhizosphere soil bacterial assembly between tomatoes and peppers infected by Ralstonia
作者:Yan, Mengyuan;Wu, Meng;Liu, Ming;Liu, Kai;Bao, Ying;Li, Zhongpei;Yan, Mengyuan;Wu, Meng;Liu, Ming;Liu, Kai;Bao, Ying;Li, Zhongpei;Li, Guilong;Qiu, Cunpu;Li, Zhongpei
关键词:Root exudate; Rhizosphere; Soil bacteria; Host species; Functional prediction